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Thiophene-based fluorescent probes with low cytotoxicity and high photostability for lysosomes in living cells (Articolo in rivista)

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Thiophene-based fluorescent probes with low cytotoxicity and high photostability for lysosomes in living cells (Articolo in rivista) (literal)

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Capodilupo A.L.; Vergaro V.; Baldassarre F.; Cardone A.; Corrente G.A.; Carlucci C.; Leporatti S.; Papadia P.; Gigli G.; Ciccarella G. (2015)
Thiophene-based fluorescent probes with low cytotoxicity and high photostability for lysosomes in living cells
in Biochimica et biophysica acta. G, General subjects (Print); Elsevier, Amsterdam (Paesi Bassi)
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Capodilupo A.L.; Vergaro V.; Baldassarre F.; Cardone A.; Corrente G.A.; Carlucci C.; Leporatti S.; Papadia P.; Gigli G.; Ciccarella G. (literal)

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Istituto Nanoscienze - CNR, National Nanotechnology Laboratory (NNL), Via Arnesano, Lecce, 73100, Italy; Centro di Ricerche 2HE, Università del Salento, Via Monteroni, Lecce, 73100, Italy; Istituto di Chimica dei Composti OrganoMetallici (ICCOM), Consiglio Nazionale Delle Ricerche CNR, Via Orabona, Bari, 4-70125, Italy; Università Della Calabria, via Pietro Bucci, Arcavacata di Rende, Cosenza, 87036, Italy; Dipartimento di Scienze e Tecnologie Biologiche ed Ambientali, Università del Salento, Prov. Le Lecce-Monteroni - Centro Ecotekne, Lecce, 73100, Italy; Center for Biomolecular Nanotechnologies (CBN), Fondazione Istituto Italiano di Tecnologia (IIT), Via Barsanti 1, Arnesano, 73010, Italy; Dipartimento di Matematica e Fisica Ennio de Giorgi, Università del Salento, Via Monteroni, Lecce, 73100, Italy; Dipartimento di Ingegneria dell'Innovazione, Università del Salento, Via Monteroni, Lecce, 73100, Italy (literal)

Titolo

Thiophene-based fluorescent probes with low cytotoxicity and high photostability for lysosomes in living cells (literal)

Abstract

Background: Selective imaging of lysosomes by fluorescence microscopy using specific fluorescent probes allows the study of biological processes and it is potentially useful also for diagnosis. Lysosomes are involved in numerous physiological processes, such as bone and tissue remodeling, plasma membrane repair, and cholesterol homeostasis, along with cell death and cell signaling. Despite the great number of dyes available today on the market, the search for new fluorescent dyes easily up-taken by cells, biocompatible and bearing bright and long-lasting fluorescence is still a priority. Methods: Two thiophene-based fluorescent dyes, TC1 and TC2, were synthetized as lysosome-specific probes. Results: The new dyes showed high selectivity for fluorescent staining and imaging of lysosomes and disclosed high photostability, low toxicity and pH insensitivity in the range 2-10. Conclusions: The TC dyes exhibited high co-localization coefficients (>95%) and moderate quantum yields. They showed high biocompatibility and long-term retention, important features for biological applications. General significance: The results of the present work disclose a new class of organic dyes with potential wide applications as specific and efficient lysosome probes in the study of various biological processes. (literal)

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