http://www.cnr.it/ontology/cnr/individuo/prodotto/ID297528
Microsatellite and biochemica characterization in pepper (Abstract/Poster in atti di convegno)
- Type
- Label
- Microsatellite and biochemica characterization in pepper (Abstract/Poster in atti di convegno) (literal)
- Anno
- 2014-01-01T00:00:00+01:00 (literal)
- Alternative label
Fratianni F., Tranchida-Lombardo V., Nazzaro F., Grillo S., Tucci M. (2014)
Microsatellite and biochemica characterization in pepper
in Proceedings of the 58th Italian Society of Agricultural Genetics Annual Congress, Alghero, Italy, 15/18 September 2014
(literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Fratianni F., Tranchida-Lombardo V., Nazzaro F., Grillo S., Tucci M. (literal)
- Note
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- Istituto di Scienze dell'alimentazione (ISA) - CNR Avellino
Istituto di Bioscienze e Biorisorse (IBBR) - CNR Portici (literal)
- Titolo
- Microsatellite and biochemica characterization in pepper (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#isbn
- 978-88-904570-4-3 (literal)
- Abstract
- Sweet Pepper, a species belonging to the genus Capsicum, is among the most important crops
in the Solanaceae family. Along the Italian peninsula sweet Pepper is a traditional crop,
characterised by an extraordinary variety of local landraces. Assessing the genetic diversity, the
genetic profile and the phylogenetic relationships of autochthonous Pepper varieties of the
Campania region are important steps towards the development of conservation plans, especially at a
regional scale. In the framework of a project for the preservation of local landraces threatened with
extinction, funded by the PSR 2007-2013 of the Campania Regional Council, genetic relationships
and true cultivar identity of several accessions of the main Pepper traditional cultivars of the
Campania Region (Southern Italy) were characterized using 16 published specific microsatellite
molecular (SSR) markers.
Furthermore, to better define the same traditional varieties, we analysed some biochemical
parameters including total polyphenols content (using the Folin-Ciocalteau reagent), antioxidant
activity (measuring the capability to inhibit the stable radical DPPH), and beta carotene content
evaluated through colorimetric method.
We characterized 20 Pepper local varieties with 22 accessions and identified alleles
originating the same genetic pattern for every accession of each investigated variety. Out of all the
tested markers, only 7 showed polymorphism among landraces, in accordance with the scarce
genetic variability of cultivated germplasm. Analysis of the biochemical profile revealed some
differences among varieties for all parameters. Molecular biology techniques and biochemical
profiling offer powerful analytical tools for variety identification of synonymic cultivars and could
be used for the establishment of germplasm collections with maximum genetic variability, with
positive consequences on the conservation and valorisation of the great genetic variation of
vegetable species of the Campania Region. (literal)
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