In vitro characterization of 6-Coumarin loaded solid lipid nanoparticles and their uptake by immunocompetent fish cells (Articolo in rivista)

Type
Label
  • In vitro characterization of 6-Coumarin loaded solid lipid nanoparticles and their uptake by immunocompetent fish cells (Articolo in rivista) (literal)
Anno
  • 2015-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1016/j.colsurfb.2015.01.022 (literal)
Alternative label
  • Adriana Trapani and Delia Mandracchia and Cinzia Di Franco and Héctor Cordero, Patricia Morcillo, Roberto Comparelli, Alberto Cuesta, Maria Angeles Esteban (2015)
    In vitro characterization of 6-Coumarin loaded solid lipid nanoparticles and their uptake by immunocompetent fish cells
    in Colloids and surfaces. B, Biointerfaces (Print)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Adriana Trapani and Delia Mandracchia and Cinzia Di Franco and Héctor Cordero, Patricia Morcillo, Roberto Comparelli, Alberto Cuesta, Maria Angeles Esteban (literal)
Pagina inizio
  • 79 (literal)
Pagina fine
  • 88 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url
  • http://www.sciencedirect.com/science/article/pii/S0927776515000296 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 127 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
  • 0 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Univ. Bari CNR-IPCF CNR-IFN (literal)
Titolo
  • In vitro characterization of 6-Coumarin loaded solid lipid nanoparticles and their uptake by immunocompetent fish cells (literal)
Abstract
  • Abstract The primary aim of the present work was to evaluate the in vitro uptake of 6-Coumarin (6COUM) loaded solid lipid nanoparticles (SLN) by two gilthead seabream (Sparus aurata L.) cell types: an established cell line (SAF-1 cells) and the primary cultures of head-kidney (HK)--the main haemopoietic organ in fish, equivalent to mammalian bone marrow--leucocytes. For this purpose, after the physicochemical characterization of SLN, the uptake by those immunocompetent fish cells was evaluated using flow cytometry and confocal microscopy. Concomitantly, the uptake of 6-COUM loaded {SLN} was compared with that achieved with 6-COUM loaded pectin microparticles (MPs), which were selected as a competitor of the delivery carriers. After {SLN} and {MP} physicochemical characterization, the results demonstrated that SAF-1 cells were able to internalize high percentages of 6-COUM {SLNs} when incubated for 4, 8 and 24 h, with the highest {SLN} concentration tested (10 ?g/ml). The ability of {HK} leucocytes to internalize {SLN} was also found to vary depending on both incubation time and {SLN} concentration. The highest values of {HK} leucocytes internalizing {SLN} particles (around 16%) were detected at the maximum {SLN} concentration (20 ?g/ml) at incubation times of 4 or 8 h. Conversely, {HK} leucocytes were unable to internalize {MPs} at any tested concentration and incubation time. A possible mechanism explaining the uptake into cells is proposed. The present work constitutes the first approximation to consider {SLN} as nanocarriers for delivering biologically active substances to fish. (literal)
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