The peculiar N- and C-termini of trichogin GA IV are needed for membrane interaction and human cell death induction at doses lacking antibiotic activity (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• The peculiar N- and C-termini of trichogin GA IV are needed for membrane interaction and human cell death induction at doses lacking antibiotic activity (Articolo in rivista) (literal)

Anno

• 2015-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1016/j.bbamem.2014.10.005 (literal)

Alternative label

• Tavano, Regina; Malachin, Giulia; De Zotti, Marta; Peggion, Cristina; Biondi, Barbara; Formaggio, Fernando; Papini, Emanuele (2015)
  The peculiar N- and C-termini of trichogin GA IV are needed for membrane interaction and human cell death induction at doses lacking antibiotic activity
  in Biochimica et biophysica acta. Biomembranes
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Tavano, Regina; Malachin, Giulia; De Zotti, Marta; Peggion, Cristina; Biondi, Barbara; Formaggio, Fernando; Papini, Emanuele (literal)

Pagina inizio

• 134 (literal)

Pagina fine

• 144 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 1848 (literal)

Rivista

• Biochimica et biophysica acta. Biomembranes (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

• 11 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 1 (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Univ Padua; Univ Padua (literal)

Titolo

• The peculiar N- and C-termini of trichogin GA IV are needed for membrane interaction and human cell death induction at doses lacking antibiotic activity (literal)

Abstract

• Peptaibiotics, non-ribosomally synthetized peptides from various ascomycetes, are uniquely characterized by di-allcylated alpha-amino acids, a rigid helical conformation, and membrane permeation properties. Although generally considered as antimicrobial peptides, peptaibiotics may display other toxicological properties, and their function is in many cases unknown. With the goal to define the biological activity and selectivity of the peptaibiotic trichogin GA IV from the human opportunist Trichodenna longibrachiatum we analyzed its membrane interaction, cytotoxic activity and antibacterial effect. Trichogin GA IV effectively killed several types of healthy and neoplastic human cells at doses (EC 50% = 4-6 mu M) lacking antibiotic effects on both Gram(-) and Gram(+) bacteria (MIC > 64 mu M). The peptaibiotic distinctive C-terminal primary alcohol was found to cooperate with the N-terminal n-octanoyl group to permeate the membrane phospholipid bilayer and to mediate effective binding and active endocytosis of trichogin GA IV in eukaryotic cells, two steps essential for cell death induction. Replacement of one Gly with Lys plus the simultaneous esterification of the C-terminus, strongly increased trichogin GA IV anti-Gram(+) activity (MIC 1-4 mu M), but further mitigated its cytotoxicity on human cells. (C) 2014 Elsevier B.V. All rights reserved. (literal)

Prodotto di

• Peptidi da amminoacidi non proteici con potenzialità in nanomedicina (PM.P01.021.001) (Modulo)
• Istituto di chimica biomolecolare (ICB) (Istituto)

Autore CNR

• FERNANDO FORMAGGIO (Unità di personale esterno)
• BARBARA BIONDI (Unità di personale interno)

Insieme di parole chiave

• Parole chiave di "The peculiar N- and C-termini of trichogin GA IV are needed for membrane interaction and human cell death induction at doses lacking antibiotic activity" (Insieme di parole chiave)

Incoming links:

Autore CNR di

• BARBARA BIONDI (Unità di personale interno)
• FERNANDO FORMAGGIO (Unità di personale esterno)

Prodotto

• Istituto di chimica biomolecolare (ICB) (Istituto)
• Peptidi da amminoacidi non proteici con potenzialità in nanomedicina (PM.P01.021.001) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Biochimica et biophysica acta. Biomembranes (Rivista)

Insieme di parole chiave di

• Parole chiave di "The peculiar N- and C-termini of trichogin GA IV are needed for membrane interaction and human cell death induction at doses lacking antibiotic activity" (Insieme di parole chiave)