Investigating the ability of beneficial rhizosphere microorganisms to alleviate plant water deficit using the model system tomato-Trichoderma longibrachiatum by whole-genome transcriptomics (Abstract/Poster in atti di convegno)

Type
Label
  • Investigating the ability of beneficial rhizosphere microorganisms to alleviate plant water deficit using the model system tomato-Trichoderma longibrachiatum by whole-genome transcriptomics (Abstract/Poster in atti di convegno) (literal)
Anno
  • 2014-01-01T00:00:00+01:00 (literal)
Alternative label
  • Monica De Palma, Maria Salzano, Michelina Ruocco, Pasquale Giorio, Rossella Albrizio, Gianpiero Guida, Matteo Lorito, Marina Tucci (2014)
    Investigating the ability of beneficial rhizosphere microorganisms to alleviate plant water deficit using the model system tomato-Trichoderma longibrachiatum by whole-genome transcriptomics
    in The 11th Solanaceae Conference SOL 2014, Porto Seguro, Brasil, November 2-6 2014
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Monica De Palma, Maria Salzano, Michelina Ruocco, Pasquale Giorio, Rossella Albrizio, Gianpiero Guida, Matteo Lorito, Marina Tucci (literal)
Note
  • Poster (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • CNR, Institute of Biosciences and Bioresources (IBBR), Research Division Portici, 80055 Portici (NA), Italy; CNR, Institute of Sustainable Plant Protection (IPSP), 80055 Portici (NA), Italy; CNR, Institute for Agricultural and Forestry Systems in the Mediterranean, (ISAFOM), 80056 Ercolano (NA), Italy Dipartimento di Agraria, Università degli Studi di Napoli Federico II, 80055 Portici (Na), Italy (literal)
Titolo
  • Investigating the ability of beneficial rhizosphere microorganisms to alleviate plant water deficit using the model system tomato-Trichoderma longibrachiatum by whole-genome transcriptomics (literal)
Abstract
  • The need for contrasting soil degradation while improving crop quality and productivity in the light of climate change has attracted considerable attention on the utilisation of rhizosphere microorganisms as bio-stimulants, which could contribute to convert conventional agricultural practices to environmentally sustainable management of crops. To this end, soil fungi belonging to the Trichoderma genus appear very promising since they are known to be efficient \"plant growth promoters\" and recent data indicate they could also improve plant resistance to water deficit, at least in part by enhancing the antioxidant machinery. However, the mechanisms by which they exert this effect need extensive characterisation. The model system of the facultative symbiosis between Solanum lycopersicum L. and T. longibrachiatum was therefore chosen to study the contribution of beneficial soil microorganisms to plant stress tolerance. In fact, the availability of advanced genomic platforms for both the plant and the microbe counterparts hold promises to allow a better understanding of the plant response to beneficial microorganisms and dissection of the complexity of the molecular events occurring in tomato in response to water stress. A hydroponic floating system was specifically designed which allowed interaction between tomato plants cv \"Crovarese\" and T. longibrachiatum strain MK1 to occur. Water deficiency was mimicked in three-week-old plants treated (T) and not treated (control, Ctrl) with T. longibrachiatum MK1 by adding 15% polyethylene glycol (PEG) to the growing nutrient medium for 24, 48 and 72 h, followed by a recovery phase of 7 d in fresh medium without PEG. Large-scale transcriptomic data were produced from control (Ctrl), MK1-treated (T), PEG-treated (P) and MK1+PEG-treated (T+P) tomato leaves by a directional single-end sequencing with Illumina HiSeq1500. Biometric and physiological analyses confirmed the positive effects of MK1 on growth promotion of tomato plants. As expected, PCA analysis of transcriptomic data suggests that the PEG treatment is the major cause of difference between the samples. When the fold change threshold was set to 1.1 (log2 scale), PEG was found to modify transcription of 2749 genes in respect to Ctrl (1584 up-regulated and 1165 down-regulated), while 1174 genes (540 up-regulated and 634 down-regulated) responded to MK1 As a whole, biometric, physiological and transcriptomic data indicate significant effects of MK1 on tomato response to water deficit that will be discussed in detail. This work was partly supported by the Project GenoPOM-pro PON02_00395_3082360 of the Italian Ministry of Education, University and Scientific Research (MIUR). (literal)
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