Sperm motility and fertilization performance of Nodipecten nodosus (L., 1758) exposed at two different cryoprotectants (Articolo in rivista)

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  • Sperm motility and fertilization performance of Nodipecten nodosus (L., 1758) exposed at two different cryoprotectants (Articolo in rivista) (literal)
Anno
  • 2014-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1111/are.12521 (literal)
Alternative label
  • Silvestri F., Vitiello V., Del Prete F., Fabbrocini A., Ferreira J.F., Sansone G. (2014)
    Sperm motility and fertilization performance of Nodipecten nodosus (L., 1758) exposed at two different cryoprotectants
    in Aquaculture research (Print); Blackwell Scientific Publications, Oxford (Regno Unito)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Silvestri F., Vitiello V., Del Prete F., Fabbrocini A., Ferreira J.F., Sansone G. (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url
  • http://onlinelibrary.wiley.com/doi/10.1111/are.12521/pdf (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali
  • 8 (literal)
Note
  • Scopus (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • FIPERJ - Fundacao Instituto de Pesca do Estado do Rio de Janeiro, Angra dos Reis, Brazil Laboratorio di Fisiologia Ambientale e degli Organismi Acquatici & Laboratorio di Criobiologia, Universita degli studi di Napoli Federico II, Napoli, Italy Istituto di Scienze Marine - UOS Lesina, Consiglio Nazionale delle Ricerche, Lesina, Italy Laboratorio de Moluscos Marinhos, Universidade Federal de Santa Catarina, Florian opolis, Brazil (literal)
Titolo
  • Sperm motility and fertilization performance of Nodipecten nodosus (L., 1758) exposed at two different cryoprotectants (literal)
Abstract
  • Cryopreservation is a valuable tool for aquaculture as it provides continuous seed production, regardless of the spawning season of the brood stock. The selection of a suitable cryoprotectant with low toxicity and high water solubility is important to avoid membrane injuries and intracellular ice crystallization. This study has been aimed at the assessment of the toxic effects of two usually applied cryoprotectants, 1-2 propylene glycol (PG) and methanol (MetOH), on spermatozoa of the of lion-paw scallop Nodipecten nodosus, by evaluating the sperm motility and the development of D larvae after fertilization procedure. Sperm was exposed at room temperature (22°C) for 10, 20 and 30 min to different concentration ranges of two cryoprotectants. Regarding the sperm motility, PG5%, PG7%, MetOH4% and MetOH6% did not show differences compared to control (semen incubated in seawater) (P < 0.05). The development of D larvae was not affected by the exposition to PG5%, MetOH 4% and MetOH 6%. These results indicate the potential use of both cryoprotectants for cryopreservation procedures. (literal)
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