Galactose grafting on poly(?-caprolactone) substrate for tissue engineering: a preliminary study (Articolo in rivista)

Type
Label
  • Galactose grafting on poly(?-caprolactone) substrate for tissue engineering: a preliminary study (Articolo in rivista) (literal)
Anno
  • 2014-01-01T00:00:00+01:00 (literal)
Alternative label
  • Laura Russo, Teresa Russo, Chiara Battocchio, Francesca Taraballi, Antonio Gloria, Ugo D'Amora, Roberto De Santis, Giovanni Polzonetti, Francesco Nicotra, Luigi Ambrosio, Laura Cipolla (2014)
    Galactose grafting on poly(?-caprolactone) substrate for tissue engineering: a preliminary study
    in Carbohydrate research (Print)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Laura Russo, Teresa Russo, Chiara Battocchio, Francesca Taraballi, Antonio Gloria, Ugo D'Amora, Roberto De Santis, Giovanni Polzonetti, Francesco Nicotra, Luigi Ambrosio, Laura Cipolla (literal)
Rivista
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  • Dept. of Biotechnology and Biosciences, University of Milano-Bicocca, Piazza della Scienza 2, 20126 Milano, Italy Institute of Polymers, Composites and Biomaterials--National Research Council of Italy, Viale J.F. Kennedy 54, Mostra d'Oltremare--PAD. 20, 80125 Naples, Italy Department of Sciences, INSTM, CNISM and CISDiC, University Roma Tre, Via della Vasca Navale 79, 00146 Rome, Italy (literal)
Titolo
  • Galactose grafting on poly(?-caprolactone) substrate for tissue engineering: a preliminary study (literal)
Abstract
  • The grafting of galactose units onto poly(e-caprolactone) (PCL) substrates by a wet chemistry two-step procedure is proposed. Even though a reduction of hardness from 0.58-0.31 GPa to 0.12-0.05 GPa is achieved, the chemical functionalization does not negatively affect the tensile modulus (332.2 ± 31.3 MPa and 328.5 ± 34.7 MPa for unmodified and surface-modified PCL, respectively) and strength (15.1 ± 1.3 MPa and 14.8 ± 1.5 MPa as assessed before and after the surface modification, respec- tively), as well as the mechanical behaviour evaluated through small punch test. XPS and enzyme-linked lectin assay (ELLA) demonstrate the presence, and also the correct exposition of the saccharidic epitope on PCL substrates. The introduction of carbohydrate moieties on the PCL surfaces clearly enhances the hydrophilicity of the substrate, as the water contact angle decreases from 82.1 ± 5.8? to 62.1 ± 4.2?. Furthermore, preliminary biological analysis shows human mesenchymal stem cell viability over time and an improvement of cell adhesion and spreading. (literal)
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