http://www.cnr.it/ontology/cnr/individuo/prodotto/ID291853

Lipase biofilm deposited by Matrix Assisted Pulsed Laser Evaporation technique (Articolo in rivista)

Type

Label

Lipase biofilm deposited by Matrix Assisted Pulsed Laser Evaporation technique (Articolo in rivista) (literal)

Anno

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

Alternative label

Antonio Aronne a, Francesco Bloisi b,c, Raffaela Calabria d, Valeria Califano d,Laura E. Depero e, Esther Fanelli a, Stefania Federici e, Patrizio Massoli d,Luciano R.M. Vicari b,c (2014)
Lipase biofilm deposited by Matrix Assisted Pulsed Laser Evaporation technique
in Applied surface science
(literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

Antonio Aronne a, Francesco Bloisi b,c, Raffaela Calabria d, Valeria Califano d,Laura E. Depero e, Esther Fanelli a, Stefania Federici e, Patrizio Massoli d,Luciano R.M. Vicari b,c (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url

Rivista

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

a Department of Chemical Engineering, Materials and Industrial Production, University of Naples \"Federico II\", Napoli, Italy b SPIN - CNR, Naples, Italy c Department of Physics, University of Naples \"Federico II\", Napoli, Italy d Istituto Motori - CNR, Naples, Italy e Department of Mechanical and Industrial Engineering, University of Brescia, Brescia, Italy (literal)

Titolo

Lipase biofilm deposited by Matrix Assisted Pulsed Laser Evaporation technique (literal)

Abstract

tLipase is an enzyme that finds application in biodiesel production and for detection of esters and tri-glycerides in biosensors. Matrix Assisted Pulsed Laser Evaporation (MAPLE), a technique derived fromPulsed Laser Deposition (PLD) for deposition of undamaged biomolecules or polymers, is characterizedby the use of a frozen target obtained from a solution/suspension of the guest material (to be deposited)in a volatile matrix (solvent). The presence of the solvent avoids or at least reduces the potential damageof guest molecules by laser radiation but only the guest material reaches the substrate in an essentiallysolvent-free deposition. MAPLE can be used for enzymes immobilization, essential for industrial applica-tion, allowing the development of continuous processes, an easier separation of products, the reuse of thecatalyst and, in some cases, enhancing enzyme properties (pH, temperature stability, etc.) and catalyticactivity in non-aqueous media. Here we show that MAPLE technique can be used to deposit undamagedlipase and that the complex structure (due to droplets generated during extraction from target) of thedeposited material can be controlled by changing the laser beam fluence. (literal)

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