NMR metabolomic analysis of exhaled breath condensate of asthmatic patients at two different temperatures (Articolo in rivista)

Type
Label
  • NMR metabolomic analysis of exhaled breath condensate of asthmatic patients at two different temperatures (Articolo in rivista) (literal)
Anno
  • 2014-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1021/pr5010407 (literal)
Alternative label
  • Andrea Motta 1, Debora Paris 1, Maria D'Amato 2, Dominique Melck 1, Cecilia Calabrese 3, Carolina Vitale 2, Anna A. Stanziola 2, Gaetano Corso 4, Matteo Sofia 2, and Mauro Maniscalco 2,5 (2014)
    NMR metabolomic analysis of exhaled breath condensate of asthmatic patients at two different temperatures
    in Journal of proteome research (Print)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Andrea Motta 1, Debora Paris 1, Maria D'Amato 2, Dominique Melck 1, Cecilia Calabrese 3, Carolina Vitale 2, Anna A. Stanziola 2, Gaetano Corso 4, Matteo Sofia 2, and Mauro Maniscalco 2,5 (literal)
Pagina inizio
  • 6107 (literal)
Pagina fine
  • 20 (literal)
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  • 13 (literal)
Rivista
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  • 12 (literal)
Note
  • ISI Web of Science (WOS) (literal)
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  • 1 Institute of Biomolecular Chemistry, National Research Council, Via Campi Flegrei 34, Pozzuoli (Naples) 80078, Italy 2 Department of Respiratory Medicine, A.O. Dei Colli, University of Naples Federico II, Via Leonardo Bianchi 1, Naples 80131, Italy 3 Department of Cardiovascular and Respiratory Sciences, A.O. Dei Colli, Second University of Naples SUN, Via Leonardo Bianchi 1, Naples 80131, Italy 4 Department of Clinical and Experimental Medicine, University of Foggia, Viale Luigi Pinto, Foggia 71122, Italy 5 Section of Respiratory Medicine, Hospital S. Maria della Pietà, Via San Rocco 9, Casoria (Naples) 80026, Italy (literal)
Titolo
  • NMR metabolomic analysis of exhaled breath condensate of asthmatic patients at two different temperatures (literal)
Abstract
  • Exhaled breath condensate (EBC) collection is a noninvasive method to investigate lung diseases. EBC is usually collected with commercial/custom-made condensers, but the optimal condensing temperature is often unknown. As such, the physical and chemical properties of exhaled metabolites should be considered when setting the temperature, therefore requiring validation and standardization of the collecting procedure. EBC is frequently used in nuclear magnetic resonance (NMR)-based metabolomics, which unambiguously recognizes different pulmonary pathological states. Here we applied NMR-based metabolomics to asthmatic and healthy EBC samples collected with two commercial condensers operating at -27.3 and -4.8 °C. Thirty-five mild asthmatic patients and 35 healthy subjects were included in the study, while blind validation was obtained from 20 asthmatic and 20 healthy different subjects not included in the primary analysis. We initially analyzed the samples separately and assessed the within-day, between-day, and technical repeatabilities. Next, samples were interchanged, and, finally, all samples were analyzed together, disregarding the condensing temperature. Partial least-squares discriminant analysis of NMR spectra correctly classified samples, without any influence from the temperature. Input variables were either integral bucket areas (spectral bucketing) or metabolite concentrations (targeted profiling). We always obtained strong regression models (95%), with high average-quality parameters for spectral profiling (R(2) = 0.84 and Q(2) = 0.78) and targeted profiling (R(2) = 0.91 and Q(2) = 0.87). In particular, although targeted profiling clustering is better than spectral profiling, all models reproduced the relative metabolite variations responsible for class differentiation. This warrants that cross comparisons are reliable and that NMR-based metabolomics could attenuate some specific problems linked to standardization of EBC collection. (literal)
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