Chemically modified poly(2-hydroxyethyl methacrylate) cryogel for the adsorption of heparin (Articolo in rivista)

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Label
  • Chemically modified poly(2-hydroxyethyl methacrylate) cryogel for the adsorption of heparin (Articolo in rivista) (literal)
Anno
  • 2014-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1002/jbm.b.33104 (literal)
Alternative label
  • Spina R.L.; Tripisciano C.; Mecca T.; Cunsolo F.; Weber V.; Mattiasson B. (2014)
    Chemically modified poly(2-hydroxyethyl methacrylate) cryogel for the adsorption of heparin
    in Journal of biomedical materials research. Part B, Applied biomaterials
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Spina R.L.; Tripisciano C.; Mecca T.; Cunsolo F.; Weber V.; Mattiasson B. (literal)
Pagina inizio
  • 1207 (literal)
Pagina fine
  • 1216 (literal)
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  • http://www.scopus.com/inward/record.url?eid=2-s2.0-84904045631&partnerID=q2rCbXpz (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 102 (literal)
Rivista
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  • 10 (literal)
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  • 6 (literal)
Note
  • Scopu (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Department of Biotechnology, Lund University, SE-221 00 Lund, Sweden; Department for Health Sciences and Biomedicine, Center for Biomedical Technology, Danube University Krems, 3500 Krems, Austria; Christian Doppler Laboratory for Innovative Therapy Approaches in Sepsis, Danube University Krems, 3500 Krems, Austria; Institute of Biomolecular Chemistry, National Research Council, 95126 Catania, Italy; EC Joint Research Centre, NBS Unit Varese, Via E. Fermi 2749, 21027 Ispra, Italy (literal)
Titolo
  • Chemically modified poly(2-hydroxyethyl methacrylate) cryogel for the adsorption of heparin (literal)
Abstract
  • Various clinical procedures, such as cardiovascular surgery or extracorporeal blood purification, involve systemic anticoagulation using heparin. High concentrations of circulating heparin require neutralization due to possible serious bleeding complications. The intravenous administration of the heparin antagonist protamine sulfate is routinely clinically performed, but is frequently associated with adverse reactions. Therefore, there is a need for a valid and safe alternative to achieve extracorporeal heparin removal from blood or plasma, such as a filter, a matrix, or an adsorbent. Here, we describe the development of a macroporous poly(2-hydroxyethyl methacrylate)-based monolithic cryogel functionalized with l-lysine (pHEMA-lys) and the characterization of its selective heparin adsorption. The maximum binding capacity was quantified in vitro using aqueous and serum solutions under static and dynamic conditions, and fresh human plasma under static conditions. The pHEMA-lys bound 40,500 IU and 32,500 IU heparin/g cryogel at the equilibrium in aqueous solution and 50% serum, respectively. In human plasma spiked with 100 IU/mL of heparin, the binding was still highly efficient (4330 IU/g cryogel after 30 min, i.e., 87% of the initial concentration). The cryogels showed good blood compatibility, as indicated by negligible adsorption of albumin, antithrombin III, and total protein, and may thus be suitable for extracorporeal heparin removal. © 2014 Wiley Periodicals, Inc. (literal)
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