Identification of Prototheca species responsible for bovine mastitis by new multiplex PCR method (Abstract/Poster in atti di convegno)

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Label
  • Identification of Prototheca species responsible for bovine mastitis by new multiplex PCR method (Abstract/Poster in atti di convegno) (literal)
Anno
  • 2014-01-01T00:00:00+01:00 (literal)
Alternative label
  • Emanuele Capra1, Paola Cremonesi1, Claudia Cortimiglia2, Giulia Bignoli1, Matteo Ricchi3, Mario Luini2, Paolo Moroni4,5, and Bianca Castiglioni1 (2014)
    Identification of Prototheca species responsible for bovine mastitis by new multiplex PCR method
    in National Mastitis Council (NMC), Worthington Renaissance Hotel Fort Worth, Texas, January 26-28, 2014
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Emanuele Capra1, Paola Cremonesi1, Claudia Cortimiglia2, Giulia Bignoli1, Matteo Ricchi3, Mario Luini2, Paolo Moroni4,5, and Bianca Castiglioni1 (literal)
Note
  • Abstract (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • 1Institute of Agricultural Biology and Biotechnology, Milan, Italy 2Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia, Lodi, Italy 3Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia, Piacenza, Italy 4Università degli Studi di Milano, Dipartimento di Scienze Veterinarie per la Salute, la Produzione Animale e la Sicurezza Alimentare, Milan, Italy 5Cornell University, Animal Health Diagnostic Center, Quality Milk Production Services, 240 Farrier Road, Ithaca, NY, 14853, USA15 (literal)
Titolo
  • Identification of Prototheca species responsible for bovine mastitis by new multiplex PCR method (literal)
Abstract
  • Within the known Prototheca spp., P. zopfii genotype 2, P. wickerhamii and P. blaschkeae are algal pathogens associated with pathology in humans and animals. Different molecular techniques have been proposed to identify these pathogens, however such of these methods are unable to differentiate among different Prototheca species or are time consuming, demanding more than a PCR reaction. The purpose of the present study was to developed a multiplex PCR reaction, based on specific amplification of mithocondrial and chloroplastic sequences, able to identify in a single reaction the three Prototheca spp. causing bovine mastitis. Between the 30 strains collected from IMI milk samples and bulk tank milk, our preliminary results, obtained by this rapid and specific assay, showed an high prevalence of P. zopfii genotype 2 in Italian herd. (literal)
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