Simultaneous identification by multiplex PCR of major Prototheca spp. isolated from bovine and buffalo intramammary infection and bulk tank. (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Simultaneous identification by multiplex PCR of major Prototheca spp. isolated from bovine and buffalo intramammary infection and bulk tank. (Articolo in rivista) (literal)

Anno

• 2014-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1111/lam.12326 (literal)

Alternative label

• Capra, E; Cremonesi, P; Cortimiglia, C; Bignoli, G; Ricchi, M; Moroni, P; Pesce, A; Luini, M; Castiglioni, B (2014)
  Simultaneous identification by multiplex PCR of major Prototheca spp. isolated from bovine and buffalo intramammary infection and bulk tank.
  in Letters in applied microbiology
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Capra, E; Cremonesi, P; Cortimiglia, C; Bignoli, G; Ricchi, M; Moroni, P; Pesce, A; Luini, M; Castiglioni, B (literal)

Pagina inizio

• 642 (literal)

Pagina fine

• 647 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 59 (literal)

Rivista

• Letters in applied microbiology (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 6 (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• IBBA-CNR: Capra, Cremonesi, Bignoli, Castiglioni IZSLER: Cortimiglia, Ricchi, Pesce, Luini UNIMI: Moroni Cornell University - USA: Moroni (literal)

Titolo

• Simultaneous identification by multiplex PCR of major Prototheca spp. isolated from bovine and buffalo intramammary infection and bulk tank. (literal)

Abstract

• Bovine mastitis caused by Prototheca spp. infection is increasing worldwide, therefore becoming more relevant to the dairy industry. Almost all Prototheca isolates from bovine mammary protothecosis came from P. zopfii genotype 2, with a lower prevalence of infection due to P. blaschkeae and rarely to P. wickerhamii. In this study, we report the development of two multiplex PCR assays able to discriminate among the three species responsible for bovine intramammary infection (IMI). Our assay is based on the specific amplification of new DNA target from mitochondria and chloroplasts partial sequences, of different Prototheca isolates. Both methods were set up using reference strains belonging to all Prototheca species and validated by the analysis of 93 isolates from bovine and buffalo IMI and bulk tank milk samples. The investigation involves 70 isolates from North, 13 from Central and 10 from South Italian regions. Isolates from bovine were most commonly identified as P. zopfii genotype 2, and only in one case as P. blaschkeae, whereas isolates from buffaloes belonged both to P. zopfii genotype 2 and P. wickerhamii. These findings proved the suitability of our multiplex PCRs as a rapid test to discriminate among pathogenic Prototheca strains. (literal)

Prodotto di

• Institute of agricultural biology and biotechnology (IBBA) (Istituto)
• Conservazione delle risorse genetiche animali e valorizzazione delle loro produzioni (AG.P01.020.001) (Modulo)

Autore CNR

• BIANCA MARIA ELISABETTA CASTIGLIONI (Unità di personale interno)
• PAOLA CREMONESI (Unità di personale interno)
• EMANUELE CAPRA (Persona)
• GIULIA BIGNOLI (Persona)

Insieme di parole chiave

• Keywords of "Simultaneous identification by multiplex PCR of major Prototheca spp. isolated from bovine and buffalo intramammary infection and bulk tank." (Insieme di parole chiave)

Incoming links:

Prodotto

• Institute of agricultural biology and biotechnology (IBBA) (Istituto)
• Conservazione delle risorse genetiche animali e valorizzazione delle loro produzioni (AG.P01.020.001) (Modulo)

Autore CNR di

• BIANCA MARIA ELISABETTA CASTIGLIONI (Unità di personale interno)
• PAOLA CREMONESI (Unità di personale interno)
• GIULIA BIGNOLI (Persona)
• EMANUELE CAPRA (Persona)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Letters in applied microbiology (Rivista)

Insieme di parole chiave di

• Keywords of "Simultaneous identification by multiplex PCR of major Prototheca spp. isolated from bovine and buffalo intramammary infection and bulk tank." (Insieme di parole chiave)