Exosomes: novel effectors of human platelet lysate activity. (Articolo in rivista)

Type
Label
  • Exosomes: novel effectors of human platelet lysate activity. (Articolo in rivista) (literal)
Anno
  • 2014-01-01T00:00:00+01:00 (literal)
Alternative label
  • Torreggiani E, Perut F, Roncuzzi L, Zini N, Baglio S R, Baldini N (2014)
    Exosomes: novel effectors of human platelet lysate activity.
    in European cells & materials
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Torreggiani E, Perut F, Roncuzzi L, Zini N, Baglio S R, Baldini N (literal)
Pagina inizio
  • 137 (literal)
Pagina fine
  • 151 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 28 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali
  • 15 (literal)
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Laboratory for Orthopaedic Pathophysiology and Regenerative Medicine, Istituto Ortopedico Rizzoli, Bologna, Italy. CNR - National Research Council, Institute of Molecular Genetics, Bologna, Italy. Laboratory of Muscoloskeletal Cell Biology, Istituto Ortopedico Rizzoli, Bologna, Italy. Department of Biomedical and Neuromotor Sciences, University of Bologna, Bologna, Italy (literal)
Titolo
  • Exosomes: novel effectors of human platelet lysate activity. (literal)
Abstract
  • Despite the popularity of platelet-rich plasma (PRP) and platelet lysate (PL) in orthopaedic practice, the mechanism of action and the effectiveness of these therapeutic tools are still controversial. So far, the activity of PRP and PL has been associated with different growth factors (GF) released during platelet degranulation. This study, for the first time, identifies exosomes, nanosized vesicles released in the extracellular compartment by a number of elements, including platelets, as one of the effectors of PL activity. Exosomes were isolated from human PL by differential ultracentrifugation, and analysed by electron microscopy and Western blotting. Bone marrow stromal cells (MSC) treated with three different exosome concentrations (0.6mug, 5mug and 50mug) showed a significant, dose-dependent increase in cell proliferation and migration compared to the control. In addition, osteogenic differentiation assays demonstrated that exosome concentration differently affected the ability of MSC to deposit mineralised matrix. Finally, the analysis of exosome protein content revealed a higher amount of basic fibroblast growth factor (bFGF), vascular endothelial growth factor (VEGF), platelet-derived growth factor (PDGF-BB) and transforming growth factor beta 1 (TGF-beta1) as compared to PL. In regards to RNA content, an enrichment of small RNAs in exosomes as compared to donor platelets has been found. These results suggest that exosomes consistently contribute to PL activity and could represent an advantageous nanodelivery system for cell-free regeneration therapies. (literal)
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