Highly sensitive immunoassay based on SERS using nano-Au immune probes and a nano-Ag immune substrate (Articolo in rivista)

Type
Label
  • Highly sensitive immunoassay based on SERS using nano-Au immune probes and a nano-Ag immune substrate (Articolo in rivista) (literal)
Anno
  • 2014-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1016/j.talanta.2014.02.015 (literal)
Alternative label
  • Shu, Lei; Zhou, Jun; Yuan, Xiaocong; Petti, Lucia; Chen, Jinping; Jia, Zhenhong; Mormile, Pasquale (2014)
    Highly sensitive immunoassay based on SERS using nano-Au immune probes and a nano-Ag immune substrate
    in Talanta (Oxf.)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Shu, Lei; Zhou, Jun; Yuan, Xiaocong; Petti, Lucia; Chen, Jinping; Jia, Zhenhong; Mormile, Pasquale (literal)
Pagina inizio
  • 161 (literal)
Pagina fine
  • 168 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 123 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali
  • 8 (literal)
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Ningbo University; Shenzhen University; Institute Cybernetics E. Caianiello CNR; Xinjiang University (literal)
Titolo
  • Highly sensitive immunoassay based on SERS using nano-Au immune probes and a nano-Ag immune substrate (literal)
Abstract
  • A super-high-sensitivity immunoassay based on surface-enhanced Raman scattering (SERS) was implemented using the nano-Au immune probes and nano-Ag immune substrate. Ultraviolet-visible extinction spectra, transmission electron microscopy (TEM) and scanning electron microscopy (SEM) images, and SERS spectra were used to characterise the nano-Au immune probes and the nano-Ag immune substrate. The nano-Ag immune substrate was prepared by the in situ growth of Ag nanoparticles and the subsequent linkage of these nanoparticles with anti-apolipoprotein B on a silicon wafer. The nano-Ag immune substrate exhibited strong SERS activity, excellent reproducibility, and high biospecificity. The nano-Au immune probes were prepared by immobilising 4-mercaptobenzoic acid (4MBA) molecules as a Raman reporter and anti-apolipoprotein B onto the surfaces of Au nanoparticles. It was found that 4MBA induced the aggregation of Au nanoparticles, resulting in the generation of vast hot spots. Moreover, the nano-Au immune probes exhibited strong SERS activity and high biospecificity. A sandwich-type immunoassay structure consisting of the nano-Au immune probes and nano-Ag immune substrate was used to detect the concentration of apolipoprotein B, where the detection limit was as low as 2 fg/mL (3.878 x 10(-18) mol/L). Taken together, the experimental results indicate that the proposed immunoassay protocol has a great potential application in biological sensing and clinical diagnostics. (c) 2014 Elsevier B.V. All rights reserved. (literal)
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