Assessment of an efficient xeno-free culture system for human periodontal ligament stem cells. (Articolo in rivista)

Type
Label
  • Assessment of an efficient xeno-free culture system for human periodontal ligament stem cells. (Articolo in rivista) (literal)
Anno
  • 2015-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1089/ten.tec.2014.0024 (literal)
Alternative label
  • Trubiani O, Piattelli A, Gatta V, Marchisio M, Diomede F, D'Aurora M, Merciaro I, Pierdomenico L, Maraldi NM, Zini N. (2015)
    Assessment of an efficient xeno-free culture system for human periodontal ligament stem cells.
    in Tissue engineering. Part C Methods
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Trubiani O, Piattelli A, Gatta V, Marchisio M, Diomede F, D'Aurora M, Merciaro I, Pierdomenico L, Maraldi NM, Zini N. (literal)
Pagina inizio
  • 52 (literal)
Pagina fine
  • 64 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 21 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali
  • 13 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
  • 1 (literal)
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Laboratory of Stem Cells and Regenerative Medicine, Department of Medical, Oral and Biotechnological Sciences, School of Medicine and Health Sciences, University \"G. d'Annunzio\" Chieti-Pescara, Chieti, Italy. Functional Genetics Unit, Department of Psychological, Humanities and Territory Sciences, School of Medicine and Health Sciences, Center of Excellence on Aging (Ce.Si.), University \"G. d'Annunzio\" Chieti-Pescara, Chieti, Italy.Department of Medicine and Aging Science, School of Medicine and Health Sciences, University \"G. d'Annunzio\" Chieti-Pescara, Chieti, Italy. Functional Genetics Unit, Department of Neuroscience and Imaging, School of Medicine and Health Sciences, Center of Excellence on Aging (Ce.Si.), University \"G. d'Annunzio\" Chieti-Pescara, Chieti, Italy. CNR-National Research Council of Italy, IGM and SC Laboratory of Musculosckeletal Cell Biology, IOR, Bologna, Italy. (literal)
Titolo
  • Assessment of an efficient xeno-free culture system for human periodontal ligament stem cells. (literal)
Abstract
  • The possibility of transplanting adult stem cells into damaged organs has opened new prospects for the treatment of several human pathologies. The purpose of this study was to develop a culture system for the expansion and production of human Periodontal Ligament Stem Cells (hPDLSCs) using a new xeno-free media formulation and ensuring the maintenance of the stem cells features comprising the multiple passage expansion, mesengenic lineage differentiation, cellular phenotype, and genomic stability, essential elements for conforming to translation to cell therapy. Somatic stem cells were isolated from the human periodontium using a minimally invasive periodontal access flap surgery in healthy donors. Expanded hPDLSCs in a xeno-free culture showed the morphological features of stem cells, expressed the markers associated with pluripotency, and a normal karyotype. Under appropriate culture conditions, hPDLSCs presented adipogenic and osteogenic potential; indeed, a very high accumulation of lipid droplets was evident in the cytoplasm of adipogenicinduced cells, and indisputable evidence of osteogenic differentiation, investigated by transmission electron microscopy, and analyzed for gene expression analysis has been shown. Based on these data, the novel xenofree culture method might provide the basis for Good Manufacturing Procedure culture of autologous stem cells, readily accessible from human periodontium, and can be a resource to facilitate their use in human clinical studies for potential therapeutic regeneration. (literal)
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