Nontemplated nucleotide additions distinguish the small RNA composition in cells from exosomes (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Nontemplated nucleotide additions distinguish the small RNA composition in cells from exosomes (Articolo in rivista) (literal)

Anno

• 2014-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1016/j.celrep.2014.08.027 (literal)

Alternative label

• Koppers-Lalic D, Hackenberg M, Bijnsdorp IV, van Eijndhoven MA, Sadek P, Sie D, Zini N, Middeldorp JM, Ylstra B, de Menezes RX, Würdinger T, Meijer GA, Pegtel DM. (2014)
  Nontemplated nucleotide additions distinguish the small RNA composition in cells from exosomes
  in Cell reports
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Koppers-Lalic D, Hackenberg M, Bijnsdorp IV, van Eijndhoven MA, Sadek P, Sie D, Zini N, Middeldorp JM, Ylstra B, de Menezes RX, Würdinger T, Meijer GA, Pegtel DM. (literal)

Pagina inizio

• 1649 (literal)

Pagina fine

• 1658 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url

• http://www.scopus.com/inward/record.url?eid=2-s2.0-84907415649&partnerID=q2rCbXpz (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 8 (literal)

Rivista

• Cell reports (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 6 (literal)

Note

• Scopu (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Department of Pathology, VU University Medical Center, Amsterdam, 1007MB, Netherlands; Exosomes Research Group, VU University Medical Center, Amsterdam, 1007MB, Netherlands; Department of Genetics, Computational Genomics and Bioinformatics Group, University of Granada, Granada, 18071, Spain; Department of Urology, VU University Medical Center, Amsterdam, 1007MB, Netherlands; CNR-National Research Council of Italy, IGM and SC Laboratory of Musculoskeletal Cell Biology, IOR, Bologna, 40136, Italy; Department of Epidemiology and Biostatistics, VU University Medical Center, Amsterdam, 1007MB, Netherlands; Department of Neurosurgery, Neuro-Oncology Research Group, VU University Medical Center, Amsterdam, 1007MB, Netherlands; Department of Neurology, Massachusetts General Hospital and Harvard Medical School, Charlestown, MA, 02129, United States (literal)

Titolo

• Nontemplated nucleotide additions distinguish the small RNA composition in cells from exosomes (literal)

Abstract

• Functional biomolecules, including small noncoding RNAs (ncRNAs), are released and transmitted between mammalian cells via extracellular vesicles (EVs), including endosome-derived exosomes. The small RNA composition in cells differs from exosomes, but underlying mechanisms have not been established. We generated small RNA profiles by RNA sequencing (RNA-seq) from a panel of human B cells and their secreted exosomes. A comprehensive bioinformatics and statistical analysis revealed nonrandomly distributed subsets of microRNA (miRNA) species between B cells and exosomes. Unexpectedly, 3' end adenylated miRNAs are relatively enriched in cells, whereas 3' end uridylated isoforms appear overrepresented in exosomes, as validated in naturally occurring EVs isolated from human urine samples. Collectively, our findings suggest that posttranscriptional modifications, notably 3' end adenylation and uridylation, exert opposing effects that may contribute, at least in part, to direct ncRNA sorting into EVs. (literal)

Prodotto di

• Bersagli molecolari per il controllo della progressione tumorale (ME.P03.012.001) (Modulo)
• Institute of molecular genetics (IGM) (Istituto)

Autore CNR

• NICOLETTA ZINI (Persona)

Incoming links:

Prodotto

• Institute of molecular genetics (IGM) (Istituto)
• Bersagli molecolari per il controllo della progressione tumorale (ME.P03.012.001) (Modulo)

Autore CNR di

• NICOLETTA ZINI (Persona)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Cell reports (Rivista)