Identification of NuRSERY, a new functional HDAC complex composed by HDAC5, GATA1, EKLF and pERK present in human erythroid cells (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Identification of NuRSERY, a new functional HDAC complex composed by HDAC5, GATA1, EKLF and pERK present in human erythroid cells (Articolo in rivista) (literal)

Anno

• 2014-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1016/j.biocel.2014.02.019 (literal)

Alternative label

• Varricchio, Lilian; Dell'Aversana, Carmela; Nebbioso, Angela; Migliaccio, Giovanni; Altucci, Lucia; Mai, Antonello; Grazzini, Giuliano; Biker, James J.; Migliaccio, Anna Rita (2014)
  Identification of NuRSERY, a new functional HDAC complex composed by HDAC5, GATA1, EKLF and pERK present in human erythroid cells
  in International journal of biochemistry & cell biology
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Varricchio, Lilian; Dell'Aversana, Carmela; Nebbioso, Angela; Migliaccio, Giovanni; Altucci, Lucia; Mai, Antonello; Grazzini, Giuliano; Biker, James J.; Migliaccio, Anna Rita (literal)

Pagina inizio

• 112 (literal)

Pagina fine

• 122 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 50 (literal)

Rivista

• International journal of biochemistry & cell biology (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

• 11 (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Hematology/Oncology, Mount Sinai School of Medicine, One Gustave L. Levy, New York, NY, USA Institute of Genetics and Biophysics, \"Adriano Buzzati Traverso\", IGB, P. Castellino 111, Italy Department of Biochemistry, Biophysics and General Pathology, Second University of Naples, L. De Crecchio 7, Naples, Italy Cell Biology and Neuroscience, Istituto Superiore di Sanita', Viale Regina Elena 299, Italy Istituto Pasteur-Fondazione Cenci Bolognetti, Dipartimento di Chimica e Tecnologie del Farmaco, Università \"Sapienza\", P.le A. Moro 5, Italy Centro Nazionale Sangue, via Giano della Bella 27, Rome, Italy Developmental and Regenerative Biology, Mount Sinai School of Medicine, One Gustave L. Levy, New York, NY, USA Hematology, Oncology and Molecular Medicine, Istituto Superiore di Sanita', Viale Regina Elena 299, Italy (literal)

Titolo

• Identification of NuRSERY, a new functional HDAC complex composed by HDAC5, GATA1, EKLF and pERK present in human erythroid cells (literal)

Abstract

• To clarify the role of HDACs in erythropoiesis, expression, activity and function of class I (HDAC1, HDAC2, HDAC3) and class Ha (HDAC4, HDAC5) HDACs during in vitro maturation of human erythroblasts were compared. During erythroid maturation, expression of HDAC1, HDAC2 and HDAC3 remained constant and activity and GATA1 association (its partner of the NuRD complex), of HDAC1 increased. By contrast, HDAC4 content drastically decreased and HDAC5 remained constant in content but decreased in activity. In erythroid cells, pull down experiments identified the presence of a novel complex formed by HDAC5, GATA1, EKLF and pERK which was instead undetectable in cells of the megakaryocytic lineage. With erythroid maturation, association among HDAC5, GATA1 and EKLF persisted but levels of pERK sharply decreased. Treatment of erythroleukemic cells with inhibitors of ERK phosphorylation reduced by >90% the total and nuclear content of HDAC5, GATA1 and EKLF, suggesting that ERK phosphorylation is required for the formation of this complex. Based on the function of class Ha HDACs as chaperones of other proteins to the nucleus and the erythroid-specificity of HDAC5 localization, this novel HDAC complex was named nuclear remodeling shuttle elythroid (NuRSERY). Exposure of erythroid cells to the class II-selective HDAC inhibitor (HDACi) APHA9 increased gamma/(gamma+beta) globin expression ratios (Mai et al., 2007), suggesting that NuRSERY may regulate globin gene expression. In agreement with this hypothesis, exposure of erythroid cells to APHA9 greatly reduced the association among HDAC5, GATA1 and EKLF. Since exposure to APHA9 did not affect survival rates or p21 activation, NuRSERY may represent a novel, possibly less toxic, target for epigenetic therapies of hemoglobinopaties and other disorders. (C) 2014 Elsevier Ltd. All rights reserved. (literal)

Prodotto di

• Identificazione di regolatori del differenziamento, della motilità e dell'apoptosi delle cellule staminali (SV.P15.008.001) (Modulo)
• Istituto di genetica e biofisica \"Adriano Buzzati Traverso\" (IGB) (Istituto)

Autore CNR

• Lucia Altucci (Unità di personale esterno)

Insieme di parole chiave

• Keywords of "Identification of NuRSERY, a new functional HDAC complex composed by HDAC5, GATA1, EKLF and pERK present in human erythroid cells" (Insieme di parole chiave)

Incoming links:

Prodotto

• Istituto di genetica e biofisica \"Adriano Buzzati Traverso\" (IGB) (Istituto)
• Identificazione di regolatori del differenziamento, della motilità e dell'apoptosi delle cellule staminali (SV.P15.008.001) (Modulo)

Autore CNR di

• Lucia Altucci (Unità di personale esterno)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• International journal of biochemistry & cell biology (Rivista)

Insieme di parole chiave di

• Keywords of "Identification of NuRSERY, a new functional HDAC complex composed by HDAC5, GATA1, EKLF and pERK present in human erythroid cells" (Insieme di parole chiave)