Imaging adherent cells in the microfluidic channel hidden by flowing RBCs as occluding objects by a holographic method (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Imaging adherent cells in the microfluidic channel hidden by flowing RBCs as occluding objects by a holographic method (Articolo in rivista) (literal)

Anno

• 2014-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1039/c4lc00290c (literal)

Alternative label

• Bianco, Vittorio; Merola, Francesco; Miccio, Lisa; Memmolo, Pasquale; Gennari, Oriella; Paturzo, Melania; Netti, Paolo Antonio; Ferraro, Pietro (2014)
  Imaging adherent cells in the microfluidic channel hidden by flowing RBCs as occluding objects by a holographic method
  in Lab on a chip (Print)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Bianco, Vittorio; Merola, Francesco; Miccio, Lisa; Memmolo, Pasquale; Gennari, Oriella; Paturzo, Melania; Netti, Paolo Antonio; Ferraro, Pietro (literal)

Pagina inizio

• 2499 (literal)

Pagina fine

• 2504 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 14 (literal)

Rivista

• Lab on a chip (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

• 6 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 14 (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• CNR-National Institute of Optics (INO), Via Campi Flegrei 34, I-80078 Pozzuoli (NA), Italy; Center for Advanced Biomaterials for Health Care, Istituto Italiano di Tecnologia, L. Barsanti e Matteucci (NA) 53 I-80125, Italy (literal)

Titolo

• Imaging adherent cells in the microfluidic channel hidden by flowing RBCs as occluding objects by a holographic method (literal)

Abstract

• Imaging through turbid media is a challenging topic. A liquid is considered turbid when dispersed particles provoke strong light scattering, thus destroying the image formation by any standard optical system. Generally, colloidal solutions belong to the class of turbid media since dispersed particles have dimensions ranging between 0.2 mu m and 2 mu m. However, in microfluidics, another relevant issue has to be considered in the case of flowing liquid made of a multitude of occluding objects, e.g. red blood cells (RBCs) flowing in veins. In such a case instead of severe scattering processes unpredictable phase delays occur resulting in a wavefront distortion, thus disturbing or even hindering the image formation of objects behind such obstructing layer. In fact RBCs can be considered to be thin transparent phase objects. Here we show that sharp amplitude imaging and phase-contrast mapping of cells hidden behind biological occluding objects, namely RBCs, is possible in harsh noise conditions and with a large field-of view by Multi-Look Digital Holography microscopy (ML-DH). Noteworthy, we demonstrate that ML-DH benefits from the presence of the RBCs, providing enhancement in terms of numerical resolution and noise suppression thus obtaining images whose quality is higher than the quality achievable in the case of a liquid without occlusive objects. (literal)

Prodotto di

• Sviluppo di tecniche di diagnostica ottica, microscopia ed interferometria (MD.P03.022.001) (Modulo)
• Istituto di cibernetica \"Edoardo Caianiello\" (ISASI) (Istituto)

Autore CNR

• MELANIA PATURZO (Persona)
• ORIELLA GENNARI (Persona)
• LISA MICCIO (Unità di personale interno)
• Vittorio Bianco (Persona)
• FRANCESCO MEROLA (Persona)
• PIETRO FERRARO (Unità di personale interno)
• Pasquale Memmolo (Persona)

Insieme di parole chiave

• Keywords of "Imaging adherent cells in the microfluidic channel hidden by flowing RBCs as occluding objects by a holographic method" (Insieme di parole chiave)

Incoming links:

Autore CNR di

• PIETRO FERRARO (Unità di personale interno)
• MELANIA PATURZO (Persona)
• Pasquale Memmolo (Persona)
• LISA MICCIO (Unità di personale interno)
• FRANCESCO MEROLA (Persona)
• Vittorio Bianco (Persona)
• ORIELLA GENNARI (Persona)

Prodotto

• Istituto di cibernetica \"Edoardo Caianiello\" (ISASI) (Istituto)
• Sviluppo di tecniche di diagnostica ottica, microscopia ed interferometria (MD.P03.022.001) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Lab on a chip (Rivista)

Insieme di parole chiave di

• Keywords of "Imaging adherent cells in the microfluidic channel hidden by flowing RBCs as occluding objects by a holographic method" (Insieme di parole chiave)