A new 3D tracking method for Cell Mechanics investigation exploiting the capabilities of Digital Holography in Microscopy (Contributo in atti di convegno)

Type
Label
  • A new 3D tracking method for Cell Mechanics investigation exploiting the capabilities of Digital Holography in Microscopy (Contributo in atti di convegno) (literal)
Anno
  • 2014-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1117/12.2039698 (literal)
Alternative label
  • Miccio, L.; Memmolo, P.; Merola, F.; Fusco, S.; Netti, P. A.; Ferraro, P. (2014)
    A new 3D tracking method for Cell Mechanics investigation exploiting the capabilities of Digital Holography in Microscopy
    in Conference on Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII, San Francisco, CA, FEB 03-06, 2014
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Miccio, L.; Memmolo, P.; Merola, F.; Fusco, S.; Netti, P. A.; Ferraro, P. (literal)
Pagina inizio
  • 89471L (literal)
Pagina fine
  • 89471L (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#titoloVolume
  • Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 8947 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#volumeInCollana
  • 8947 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali
  • 7 (literal)
Note
  • ISI Web of Science (WOS) (literal)
  • Scopus (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • CNR - Istituto Nazionale di Ottica di Napoli, Via Campi Flegrei 34, 80078 Pozzuoli, Italy; Center for Advanced Biomaterials for Health Care at CRIB, Istituto Italiano di Tecnologia, Largo Barsanti e Matteucci 53, Napoli 80125, Italy (literal)
Titolo
  • A new 3D tracking method for Cell Mechanics investigation exploiting the capabilities of Digital Holography in Microscopy (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#isbn
  • 978-0-8194-9860-1 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#curatoriVolume
  • Farkas, DL; Nicolau, DV; Leif, RC (literal)
Abstract
  • A method for 3D tracking has been developed exploiting Digital Holography features in Microscopy (DHM). In the framework of self-consistent platform for manipulation and measurement of biological specimen we use DHM for quantitative and completely label free analysis of samples with low amplitude contrast. Tracking capability extend the potentiality of DHM allowing to monitor the motion of appropriate probes and correlate it with sample properties. Complete 3D tracking has been obtained for the probes avoiding the amplitude refocusing in traditional tracking processes. Moreover, in biology and biomedical research fields one of the main topic is the understanding of morphology and mechanics of cells and microorganisms. Biological samples present low amplitude contrast that limits the information that can be retrieved through optical bright-field microscope measurements. The main effect on light propagating in such objects is in phase. This is known as phase-retardation or phase-shift. DHM is an innovative and alternative approach in microscopy, it's a good candidate for no-invasive and complete specimen analysis because its main characteristic is the possibility to discern between intensity and phase information performing quantitative mapping of the Optical Path Length. In this paper, the flexibility of DH is employed to analyze cell mechanics of unstained cells subjected to appropriate stimuli. DHM is used to measure all the parameters useful to understand the deformations induced by external and controlled stresses on in-vitro cells. Our configuration allows 3D tracking of micro-particles and, simultaneously, furnish quantitative phase-contrast maps. Experimental results are presented and discussed for in vitro cells. (literal)
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