Effects of dental methacrylates on oxygen consumption and redox status of human pulp cells (Articolo in rivista)

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Label
  • Effects of dental methacrylates on oxygen consumption and redox status of human pulp cells (Articolo in rivista) (literal)
Anno
  • 2014-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1155/2014/956579 (literal)
Alternative label
  • Nocca G.; Calla C.; Martorana G.E.; Cicillini L.; Rengo S.; Lupi A.; Cordaro M.; Luisa Gozzo M.; Spagnuolo G. (2014)
    Effects of dental methacrylates on oxygen consumption and redox status of human pulp cells
    in BioMed Research International
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Nocca G.; Calla C.; Martorana G.E.; Cicillini L.; Rengo S.; Lupi A.; Cordaro M.; Luisa Gozzo M.; Spagnuolo G. (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url
  • http://www.scopus.com/inward/record.url?eid=2-s2.0-84896263214&partnerID=q2rCbXpz (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 2014 (literal)
Rivista
Note
  • Scopu (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Istituto di Biochimica e Biochimica Clinica, Facoltà di Medicina e Chirurgia, Università Cattolica Del Sacro Cuore, Largo Francesco Vito 1, 00168 Rome, Italy; Laboratorio di Patologia Clinica, Ospedale M.G. Vannini, Via dell'Acqua Bullicante, 00177 Rome, Italy; Department of Neurosciences, Reproductive and Odontostomatological Sciences, University of Naples Federico II, Via G. Pansini 5, 80131 Napoli, Italy; Istituto di Chimica Del Riconoscimento Molecolare, C.N.R., c/o Largo Francesco Vito 1, 00168 Rome, Italy; Istituto di Clinica Odontoiatrica, Facoltà di Medicina e Chirurgia, Università Cattolica Del Sacro Cuore, Largo Francesco Vito 1, 00168 Rome, Italy (literal)
Titolo
  • Effects of dental methacrylates on oxygen consumption and redox status of human pulp cells (literal)
Abstract
  • Several studies have already demonstrated that the incomplete polymerization of resin-based dental materials causes the release of monomers which might affect cell metabolism. The aim of this study was to investigate the effects of triethylene glycol dimethacrylate, 1,4-butanediol dimethacrylate, urethane dimethacrylate, and 2-hydroxyethyl methacrylate on (1) cellular energy metabolism, evaluating oxygen consumption rate, glucose consumption, glucose 6-phosphate dehydrogenase activity, and lactate production, and (2) cellular redox status, through the evaluation of glutathione concentration and of the activities of enzymes regulating glutathione metabolism. Methods. Human pulp cells were used and oxygen consumption was measured by means of a Clark electrode. Moreover, reactive oxygen species production was quantified. Enzymatic activity and glucose and lactate concentrations were determined through a specific kit. Results. Triethylene glycol dimethacrylate, 1,4-butanediol dimethacrylate, and 2-hydroxyethyl methacrylate induced a decrease in oxygen consumption rate, an enhancement of glucose consumption, and lactate production, whilst glucose 6-phosphate dehydrogenase and glutathione reductase activity were not significantly modified. Moreover, the monomers induced an increase of reactive oxygen species production with a consequent increase of superoxide dismutase and catalase enzymatic activities. A depletion of both reduced and total glutathione was also observed. Conclusion. The obtained results indicate that dental monomers might alter energy metabolism and glutathione redox balance in human pulp cells. © 2014 Giuseppina Nocca et al. (literal)
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