Functional Characterization of the RNA Chaperone Hfq in the Opportunistic Human Pathogen Stenotrophomonas maltophilia (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Functional Characterization of the RNA Chaperone Hfq in the Opportunistic Human Pathogen Stenotrophomonas maltophilia (Articolo in rivista) (literal)

Anno

• 2012-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1128/JB.00746-12 (literal)

Alternative label

• Roscetto, Emanuela; Angrisano, Tiziana; Costa, Valerio; Casalino, Mariassunta; Foerstner, Konrad U.; Sharma, Cynthia M.; Di Nocera, Pier Paolo; De Gregorio, Eliana (2012)
  Functional Characterization of the RNA Chaperone Hfq in the Opportunistic Human Pathogen Stenotrophomonas maltophilia
  in Journal of bacteriology (Print)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Roscetto, Emanuela; Angrisano, Tiziana; Costa, Valerio; Casalino, Mariassunta; Foerstner, Konrad U.; Sharma, Cynthia M.; Di Nocera, Pier Paolo; De Gregorio, Eliana (literal)

Pagina inizio

• 5864 (literal)

Pagina fine

• 5874 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 194 (literal)

Rivista

• Journal of bacteriology (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

• 11 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 21 (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Dipartimento di Biologia e Patologia Cellulare e Molecolare, Università Federico II, Naples, Italy Institute of Genetics and Biophysics \"Adriano Buzzati-Traverso,\" CNR, Naples, Italy Dipartimento di Biologia, Università Roma TRE, Rome, Italy Research Center for Infectious Diseases, University of Würzburg, Würzburg, Germany (literal)

Titolo

• Functional Characterization of the RNA Chaperone Hfq in the Opportunistic Human Pathogen Stenotrophomonas maltophilia (literal)

Abstract

• Hfq is an RNA-binding protein known to regulate a variety of cellular processes by interacting with small RNAs (sRNAs) and mRNAs in prokaryotes. Stenotrophomonas maltophilia is an important opportunistic pathogen affecting primarily hospitalized and immunocompromised hosts. We constructed an hfq deletion mutant (Delta hfq) of S. maltophilia and compared the behaviors of wild-type and Delta hfq S. maltophilia cells in a variety of assays. This revealed that S. maltophilia Hfq plays a role in biofilm formation and cell motility, as well as susceptibility to antimicrobial agents. Moreover, Hfq is crucial for adhesion to bronchial epithelial cells and is required for the replication of S. maltophilia in macrophages. Differential RNA sequencing analysis (dRNA-seq) of RNA isolated from S. maltophilia wild-type and Delta hfq strains showed that Hfq regulates the expression of genes encoding flagellar and fimbrial components, transmembrane proteins, and enzymes involved in different metabolic pathways. Moreover, we analyzed the expression of several sRNAs identified by dRNA-seq in wild-type and Delta hfq S. maltophilia cells grown in different conditions on Northern blots. The accumulation of two sRNAs was strongly reduced in the absence of Hfq. Furthermore, based on our dRNA-seq analysis we provide a genome-wide map of transcriptional start sites in S. maltophilia. (literal)

Prodotto di

• Plasticità genomica: dal genoma ai sistemi biologici (SV.P13.003.001) (Modulo)
• Institute of genetics and biophysics \"Adriano Buzzati Traverso\" (IGB) (Istituto)

Autore CNR

• VALERIO COSTA (Persona)

Incoming links:

Prodotto

• Institute of genetics and biophysics \"Adriano Buzzati Traverso\" (IGB) (Istituto)
• Plasticità genomica: dal genoma ai sistemi biologici (SV.P13.003.001) (Modulo)

Autore CNR di

• VALERIO COSTA (Persona)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Journal of bacteriology (Rivista)