Anion inhibition study of the beta-class carbonic anhydrase (PgiCAb) from the oral pathogen Porphyromonas gingivalis (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Anion inhibition study of the beta-class carbonic anhydrase (PgiCAb) from the oral pathogen Porphyromonas gingivalis (Articolo in rivista) (literal)

Anno

• 2014-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1016/j.bmcl.2014.08.014 (literal)

Alternative label

• Vullo, Daniela; Del Prete, Sonia; Osman, Sameh M.; Scozzafava, Andrea; Alothman, Zeid; Supuran, Claudiu T.; Capasso, Clemente (2014)
  Anion inhibition study of the beta-class carbonic anhydrase (PgiCAb) from the oral pathogen Porphyromonas gingivalis
  in Bioorganic & medicinal chemistry letters (Print)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Vullo, Daniela; Del Prete, Sonia; Osman, Sameh M.; Scozzafava, Andrea; Alothman, Zeid; Supuran, Claudiu T.; Capasso, Clemente (literal)

Pagina inizio

• 4402 (literal)

Pagina fine

• 4406 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 24 (literal)

Rivista

• Bioorganic & medicinal chemistry letters (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

• 5 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 18 (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• University of Florence; Consiglio Nazionale delle Ricerche (CNR); King Saud University; University of Florence (literal)

Titolo

• Anion inhibition study of the beta-class carbonic anhydrase (PgiCAb) from the oral pathogen Porphyromonas gingivalis (literal)

Abstract

• The oral pathogenic bacterium Porphyromonas gingivalis, encodes for two carbonic anhydrase (CA, EC 4.2.1.1) one belonging to the beta-class (PgiCAb) and another one to the gamma-class (PgiCA). This last enzyme has been characterized earlier for its inhibition profile with various classes of CA inhibitors, such as the sulfonamides and anions, whereas for PgiCAb such data were not yet reported. Here we show that PgiCAb has a good catalytic activity for the CO2 hydration reaction, with k(cat) 2.8 x 10(5) s(-1) and k(cat)/K-m of 1.5 x 10(7) M-1 x s(-1), being inhibited by cyanate and diethyldithiocarbamate in the submillimolar range (K(I)s of 0.23-0.76 mM) and more efficiently by sulfamide, sulfamate, phenylboronic acid and phenylarsonic acid (K(I)s of 60-78 mu M). The anion inhibition profile of the two P. gingivalis enzymes is very different. Identification of selective inhibitors of PgiCAb/PgiCA may lead to pharmacological tools useful for understanding the physiological role(s) of these enzymes, since this bacterium is the main causative agent of periodontitis and few treatment options are presently available. (C) 2014 Elsevier Ltd. All rights reserved. (literal)

Prodotto di

• DR. MORACCI: Studio del Rapporto Struttura-Funzione e progettazione di Enzimi e Proteine (SV.P14.004.001) (Modulo)
• Istituto di Bioscienze e Biorisorse (IBBR) (Istituto)

Autore CNR

• CLEMENTE CAPASSO (Unità di personale interno)

Insieme di parole chiave

• Parole chiave di "Anion inhibition study of the beta-class carbonic anhydrase (PgiCAb) from the oral pathogen Porphyromonas gingivalis" (Insieme di parole chiave)

Incoming links:

Autore CNR di

• CLEMENTE CAPASSO (Unità di personale interno)

Prodotto

• Istituto di Bioscienze e Biorisorse (IBBR) (Istituto)
• DR. MORACCI: Studio del Rapporto Struttura-Funzione e progettazione di Enzimi e Proteine (SV.P14.004.001) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Bioorganic & medicinal chemistry letters (Rivista)

Insieme di parole chiave di

• Parole chiave di "Anion inhibition study of the beta-class carbonic anhydrase (PgiCAb) from the oral pathogen Porphyromonas gingivalis" (Insieme di parole chiave)