Towards sensitive label-free immunosensing by means of turn-around point long period fiber gratings (Articolo in rivista)

Type
Label
  • Towards sensitive label-free immunosensing by means of turn-around point long period fiber gratings (Articolo in rivista) (literal)
Anno
  • 2014-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1016/j.bios.2014.04.042 (literal)
Alternative label
  • Chiavaioli, F.; Biswas, P.; Trono, C.; Bandyopadhyay, S.; Giannetti, A.; Tombelli, S.; Basumallick, N.; Dasgupta, K.; Baldini, F. (2014)
    Towards sensitive label-free immunosensing by means of turn-around point long period fiber gratings
    in Biosensors & bioelectronics
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Chiavaioli, F.; Biswas, P.; Trono, C.; Bandyopadhyay, S.; Giannetti, A.; Tombelli, S.; Basumallick, N.; Dasgupta, K.; Baldini, F. (literal)
Pagina inizio
  • 305 (literal)
Pagina fine
  • 310 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 60 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali
  • 6 (literal)
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Inst Appl Phys Nello Carrara; Central Glass & Ceramic Research Institute - India (literal)
Titolo
  • Towards sensitive label-free immunosensing by means of turn-around point long period fiber gratings (literal)
Abstract
  • Long period fiber gratings have been effectively used in the field of biochemical sensing since a few years. Compared to other well-known label-free optical approaches, long period gratings (LPGs) take advantage of the typical peculiarity of optical fibers. Coupling the propagating core mode with a high-order cladding mode near its turn-around point (TAP) was the strategy adopted to achieve good performances without additional coatings, except for the sensing and selective biolayer deposited on the fiber. Both the modeling and manufacturing of TAP LPGs were discussed. After the functionalization of the fiber surface with the deposition of a Eudragit L100 copolymer layer followed by immunoglobulin G (IgG) covalent immobilization, an IgG/anti-IgG bioassay was implemented along the grating region and the kinetics of antibody/antigen interaction was analyzed. A quantitative comparison between a TAP LPG and a non-TAP LPG was carried out to highlight the improvement of the proposed immunosensor. The real effectiveness and feasibility of an LPG-based biosensor were demonstrated by using a complex matrix consisting of human serum, which also confirmed the specificity of the assay, and a limit of detection of 70 mu g L-1 (460 pM) was achieved. (C) 2014 Elsevier B.V. All rights reserved. (literal)
Prodotto di
Autore CNR
Insieme di parole chiave

Incoming links:


Autore CNR di
Prodotto
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi
Insieme di parole chiave di
data.CNR.it