http://www.cnr.it/ontology/cnr/individuo/prodotto/ID284122

Involvement of calcitonin gene-related peptide and receptor component protein in experimental autoimmune encephalomyelitis (Articolo in rivista)

Type

Articolo in rivista (Classe)
Prodotto della ricerca (Classe)

Label

Involvement of calcitonin gene-related peptide and receptor component protein in experimental autoimmune encephalomyelitis (Articolo in rivista) (literal)

Anno

2014-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

10.1016/j.jneuroim.2014.03.008 (literal)

Alternative label

Sardi, Claudia; Zambusi, Laura; Finardi, Annamaria; Ruffini, Francesca; Tolun, Adviye A.; Dickerson, Ian M.; Righi, Marco; Zacchetti, Daniele; Grohovaz, Fabio; Provini, Luciano; Furlan, Roberto; Morara, Stefano (2014)
Involvement of calcitonin gene-related peptide and receptor component protein in experimental autoimmune encephalomyelitis
in Journal of neuroimmunology (Print)
(literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

Sardi, Claudia; Zambusi, Laura; Finardi, Annamaria; Ruffini, Francesca; Tolun, Adviye A.; Dickerson, Ian M.; Righi, Marco; Zacchetti, Daniele; Grohovaz, Fabio; Provini, Luciano; Furlan, Roberto; Morara, Stefano (literal)

Pagina inizio

18 (literal)

Pagina fine

29 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url

http://www.scopus.com/record/display.url?eid=2-s2.0-84900508591&origin=inward (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

271 (literal)

Rivista

Journal of neuroimmunology (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

1-2 (literal)

Note

Scopu (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

Consiglio Nazionale delle Ricerche; Universita degli Studi di Milano; Fondazione San Raffaele del Monte Tabor; University of Miami; University of Rochester; Universita Vita-Salute San Raffaele; Istituto Clinico Humanitas; Laboratory Corporation of America (literal)

Titolo

Involvement of calcitonin gene-related peptide and receptor component protein in experimental autoimmune encephalomyelitis (literal)

Abstract

Calcitonin Gene-Related Peptide (CGRP) inhibits microglia inflammatory activation in vitro. We here analyzed the involvement of CGRP and Receptor Component Protein (RCP) in experimental autoimmune encephalomyelitis (EAE).Alpha-CGRP deficiency increased EAE scores which followed the scale alpha-CGRP null. >. heterozygote. >. wild type. In wild type mice, CGRP delivery into the cerebrospinal fluid (CSF) 1) reduced chronic EAE (C-EAE) signs, 2) inhibited microglia activation (revealed by quantitative shape analysis), and 3) did not alter GFAP expression, cell density, lymphocyte infiltration, and peripheral lymphocyte production of IFN-gamma, TNF-alpha, IL-17, IL-2, and IL-4.RCP (probe for receptor involvement) was expressed in white matter microglia, astrocytes, oligodendrocytes, and vascular-endothelial cells: in EAE, also in infiltrating lymphocytes. In relapsing-remitting EAE (R-EAE) RCP increased during relapse, without correlation with lymphocyte density. RCP nuclear localization (stimulated by CGRP in vitro) was I) increased in microglia and decreased in astrocytes (R-EAE), and II) increased in microglia by CGRP CSF delivery (C-EAE). Calcitonin like receptor was rarely localized in nuclei of control and relapse mice. CGRP increased in motoneurons.In conclusion, CGRP can inhibit microglia activation in vivo in EAE. CGRP and its receptor may represent novel protective factors in EAE, apparently acting through the differential cell-specific intracellular translocation of RCP. © 2014 Elsevier B.V. (literal)

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