Impact of different ChIP-Seq protocols on DNA integrity and quality of bioinformatics analysis results (Articolo in rivista)

Type
Label
  • Impact of different ChIP-Seq protocols on DNA integrity and quality of bioinformatics analysis results (Articolo in rivista) (literal)
Anno
  • 2014-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1093/bfgp/elu001 (literal)
Alternative label
  • Felsani A.; Gudmundsson B.; Nanni S.; Brini E.; Moles A.; Thormar HG.; Estibeiro P.; Gaetano C.; Capogrossi M.; Farsetti A.; Jonsson J.J.; Guffanti A. (2014)
    Impact of different ChIP-Seq protocols on DNA integrity and quality of bioinformatics analysis results
    in Briefings in functional genomics & proteomics (Print)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Felsani A.; Gudmundsson B.; Nanni S.; Brini E.; Moles A.; Thormar HG.; Estibeiro P.; Gaetano C.; Capogrossi M.; Farsetti A.; Jonsson J.J.; Guffanti A. (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#altreInformazioni
  • 2014 Feb 21. [Epub ahead of print] (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Institute of Cell Biology and Neurobiology, CNR, Rome. (literal)
Titolo
  • Impact of different ChIP-Seq protocols on DNA integrity and quality of bioinformatics analysis results (literal)
Abstract
  • Different ChIP-Seq protocols may have a significant impact on the final outcome in terms of quality, number and distribution of called peaks. Sample DNA undergoes a long procedure before the final sequencing step, and damaged DNA can result in excessive mismatches in the alignment with reference genome. In this letter, we present the effect of well-defined modifications (timing of formaldehyde crosslink reversal, brand of the sonicator) of standard ChIP-Seq protocol on parallel samples derived from the same cell line correlating the initial DNA quality control metrics to the final bioinformatics analysis results. (literal)
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