http://www.cnr.it/ontology/cnr/individuo/prodotto/ID283557
Impact of different ChIP-Seq protocols on DNA integrity and quality of bioinformatics analysis results (Articolo in rivista)
- Type
- Label
- Impact of different ChIP-Seq protocols on DNA integrity and quality of bioinformatics analysis results (Articolo in rivista) (literal)
- Anno
- 2014-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1093/bfgp/elu001 (literal)
- Alternative label
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Felsani A.; Gudmundsson B.; Nanni S.; Brini E.; Moles A.; Thormar HG.; Estibeiro P.; Gaetano C.; Capogrossi M.; Farsetti A.; Jonsson J.J.; Guffanti A. (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#altreInformazioni
- 2014 Feb 21. [Epub ahead of print] (literal)
- Rivista
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- Institute of Cell Biology and Neurobiology, CNR, Rome. (literal)
- Titolo
- Impact of different ChIP-Seq protocols on DNA integrity and quality of bioinformatics analysis results (literal)
- Abstract
- Different ChIP-Seq protocols may have a significant impact on the final outcome in terms of quality, number and distribution of called peaks. Sample DNA undergoes a long procedure before the final sequencing step, and damaged DNA can result in excessive mismatches in the alignment with reference genome. In this letter, we present the effect of well-defined modifications (timing of formaldehyde crosslink reversal, brand of the sonicator) of standard ChIP-Seq protocol on parallel samples derived from the same cell line correlating the initial DNA quality control metrics to the final bioinformatics analysis results. (literal)
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