Transcription factor PREP1 induces EMT and metastasis by controlling the TGF-?-SMAD3 pathway in non-small cell lung adenocarcinoma. (Articolo in rivista)

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Label
  • Transcription factor PREP1 induces EMT and metastasis by controlling the TGF-?-SMAD3 pathway in non-small cell lung adenocarcinoma. (Articolo in rivista) (literal)
Anno
  • 2014-01-01T00:00:00+01:00 (literal)
Alternative label
  • Risolino M, Mandia N, Iavarone F, Dardaei L, Longobardi E, Fernandez S, Talotta F, Bianchi F, Pisati F, Spaggiari L, Harter PN, Mittelbronn M, Schulte D, Incoronato M, Di Fiore PP, Blasi F, Verde P. (2014)
    Transcription factor PREP1 induces EMT and metastasis by controlling the TGF-?-SMAD3 pathway in non-small cell lung adenocarcinoma.
    in Proceedings of the National Academy of Sciences of the United States of America
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Risolino M, Mandia N, Iavarone F, Dardaei L, Longobardi E, Fernandez S, Talotta F, Bianchi F, Pisati F, Spaggiari L, Harter PN, Mittelbronn M, Schulte D, Incoronato M, Di Fiore PP, Blasi F, Verde P. (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url
  • http://www.scopus.com/record/display.url?eid=undefined&origin=inward (literal)
Rivista
Note
  • Scopus (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Institute of Genetics and Biophysics, Consiglio Nazionale delle Ricerche, 80131 Naples, Italy; Institute of Molecular Oncology (IFOM) of the Italian Foundation for Cancer Research (FIRC), 20139 Milan, Italy; Department of Medicine, Surgery, and Dentistry, University of Milan, 20122 Milan, Italy; Neuroscience Center, Neurological Institute (Edinger Institut), 60528 Frankfurt, Germany; Istituto di Ricovero e Cura a Carattere Scientifico SDN (IRCCS SDN), 80142 Naples, Italy. (literal)
Titolo
  • Transcription factor PREP1 induces EMT and metastasis by controlling the TGF-?-SMAD3 pathway in non-small cell lung adenocarcinoma. (literal)
Abstract
  • Pre-B-cell leukemia homeobox (Pbx)-regulating protein-1 (Prep1) is a ubiquitous homeoprotein involved in early development, genomic stability, insulin sensitivity, and hematopoiesis. Previously we have shown that Prep1 is a haploinsufficient tumor suppressor that inhibits neoplastic transformation by competing with myeloid ecotropic integration site 1 for binding to the common heterodimeric partner Pbx1. Epithelial-mesenchymal transition (EMT) is controlled by complex networks of proinvasive transcription factors responsive to paracrine factors such as TGF-?. Here we show that, in addition to inhibiting primary tumor growth, PREP1 is a novel EMT inducer and prometastatic transcription factor. In human non-small cell lung cancer (NSCLC) cells, PREP1 overexpression is sufficient to trigger EMT, whereas PREP1 down-regulation inhibits the induction of EMT in response to TGF-?. PREP1 modulates the cellular sensitivity to TGF-? by inducing the small mothers against decapentaplegic homolog 3 (SMAD3) nuclear translocation through mechanisms dependent, at least in part, on PREP1-mediated transactivation of a regulatory element in the SMAD3 first intron. Along with the stabilization and accumulation of PBX1, PREP1 induces the expression of multiple activator protein 1 components including the proinvasive Fos-related antigen 1 (FRA-1) oncoprotein. Both FRA-1 and PBX1 are required for the mesenchymal changes triggered by PREP1 in lung tumor cells. Finally, we show that the PREP1-induced mesenchymal transformation correlates with significantly increased lung colonization by cells overexpressing PREP1. Accordingly, we have detected PREP1 accumulation in a large number of human brain metastases of various solid tumors, including NSCLC. These findings point to a novel role of the PREP1 homeoprotein in the control of the TGF-? pathway, EMT, and metastasis in NSCLC. (literal)
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