http://www.cnr.it/ontology/cnr/individuo/prodotto/ID283327
Soil genotoxicity assessment--results of an interlaboratory study on the Vicia micronucleus assay in the context of ISO standardization (Articolo in rivista)
- Type
- Label
- Soil genotoxicity assessment--results of an interlaboratory study on the Vicia micronucleus assay in the context of ISO standardization (Articolo in rivista) (literal)
- Anno
- 2015-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1007/s11356-014-3495-2 (literal)
- Alternative label
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Sylvie Cotelle & Adrien Dhyèvre & Serge Muller & Pascale Chenon & Nicolas Manier &
Pascal Pandard & Abdelwahad Echairi & Jérôme Silvestre & Maritxu Guiresse &
Eric Pinelli & Lucia Giorgetti & Meri Barbafieri & Valéria C. Silva & Fernanda Engel &
Claudemir M. Radetski (literal)
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- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
- Rivista
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- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- S. Cotelle (*) : A. Dhyèvre : S. Muller
Laboratoire Interdisciplinaire des Environnements Continentaux,
Université de Lorraine (LIEC), CNRSUMR 7360, 8, Rue du General
Delestraint, Metz 57070, France
e-mail: sylvie.cotelle@univ-lorraine.fr
P. Chenon
RITTMO Agro-Environnement, ZA Biopôle, 37, Rue de
Herrlisheim, Colmar 68025, France
N. Manier : P. Pandard
INERIS, Parc technologique Alata, BP2 Verneuil-en-Halatte 60550,
France
A. Echairi
WELIENCE Agro-Environnement,RD 31, Bretenière 21110, France
J. Silvestre : M. Guiresse : E. Pinelli
Université de Toulouse; INP, UPS, EcoLab (Laboratoire d'Ecologie
Fonctionnelle et Environnement ENSAT, Avenue de l'Agrobiopole,
Castanet Tolosan 31326, France
J. Silvestre : M. Guiresse : E. Pinelli
CNRS EcoLab, Castanet Tolosan 31326, France
L. Giorgetti
National Research Council (CNR), Institute of Biology and
Agricultural Biotechnology (IBBA), Research Unit of Pisa, Pisa,
Italy
M. Barbafieri
National Research Council (CNR), Institute of Ecosystem Study
(ISE), Research Unit of Pisa, Pisa, Italy
V. C. Silva : F. Engel : C. M. Radetski (*)
UNIVALI-Universidade do Vale do Itajaí, Laboratório de
Remediação Ambiental, Rua Uruguai, 458, Itajaí, SC 88302-202,
Brazil
e-mail: radetski@univali.br (literal)
- Titolo
- Soil genotoxicity assessment--results of an interlaboratory study on the Vicia micronucleus assay in the context of ISO standardization (literal)
- Abstract
- Abstract The Vicia micronucleus assay was standardized
in an international protocol, ISO 29200, \"Assessment of
genotoxic effects on higher plants--Vicia faba micronucleus
test,\" for soil or soil materials (e.g., compost, sludge,
sediment, waste, and fertilizing materials). The aim of this
interlaboratory study on the Vicia micronucleus assay was
to investigate the robustness of this in vivo assay in terms
of its applicability in different countries where each participant
were asked to use their own seeds and reference
soil, in agreement with the ISO 29200 standard. The ISO
29200 standard protocol was adopted for this study, and
seven laboratories from three countries (France, Italy, and
Brazil) participated in the study. Negative and positive
controls were correctly evaluated by 100 % of the
participants. In the solid-phase test, the micronucleus frequency
(number of micronuclei/1,000 cells) varied from
0.0 to 1.8 for the negative control (i.e., Hoagland's solution)
and from 5.8 to 85.7 for the positive control (i.e.,
maleic hydrazide), while these values varied from 0.0 to
1.7 for the negative control and from 14.3 to 97.7 for the
positive control in the case of liquid-phase test. The variability
in the data obtained does not adversely affect the
robustness of the protocol assessed, on the condition that
the methodology described in the standard ISO 29200 is
strictly respected. Thus, the Vicia micronucleus test (ISO
29200) is appropriate for complementing prokaryotic or
in vitro tests cited in legislation related to risk assessment
of genotoxicity potential. (literal)
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