T-cell responses to peptide fragments of the BK virus T antigen: implications for cross-reactivity of immune response to JC virus (Articolo in rivista)

Type
Label
  • T-cell responses to peptide fragments of the BK virus T antigen: implications for cross-reactivity of immune response to JC virus (Articolo in rivista) (literal)
Anno
  • 2006-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1099/vir.0.82094-0 (literal)
Alternative label
  • Li, Jongming; Melenhorst, Jos; Hensel, Nancy; Rezvani, Katyoun; Sconocchia, Giuseppe; Kilical, Yasemin; Hou, Jean; Curfman, Blanche; Major, Eugene; Barrett, A. John (2006)
    T-cell responses to peptide fragments of the BK virus T antigen: implications for cross-reactivity of immune response to JC virus
    in Journal of general virology (Print)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Li, Jongming; Melenhorst, Jos; Hensel, Nancy; Rezvani, Katyoun; Sconocchia, Giuseppe; Kilical, Yasemin; Hou, Jean; Curfman, Blanche; Major, Eugene; Barrett, A. John (literal)
Pagina inizio
  • 2951 (literal)
Pagina fine
  • 2960 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 87 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali
  • 10 (literal)
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • National Institutes of Health (NIH) - USA; Thomas Jefferson University; National Institutes of Health (NIH) - USA (literal)
Titolo
  • T-cell responses to peptide fragments of the BK virus T antigen: implications for cross-reactivity of immune response to JC virus (literal)
Abstract
  • Infection with BK virus (BKV) induces both humoral and cellular immunity, but the viral antigens of T-antigen (T-ag) stimulating T-cell responses are largely unknown. To identify BKV-specific T cells in healthy individuals, peripheral blood lymphocytes were cultured with autologous dendritic cells (DCs) loaded with BKV lysate and T cells were screened for intracellular gamma interferon production after stimulation with an overlapping 15mer peptide library of the BKV T-ag. Among many immunogenic peptides identified, four T-ag peptides were identified as candidate major histocompatibility complex class I and 11 T-cell epitopes, restricted to human leukocyte antigen (HLA)-B*0702, -B*08, -DRB1*0301 and -DRB1*0901. Further, a candidate 9mer peptide, LPLMRKAYL, was confirmed to be restricted to HLA-B*0702 and -B*08. Because the polyomaviruses BKV, JC virus (JCV) and Simian virus 40 (SV40) share extensive sequence similarity in the immunogenic proteins T-ag and VP1, it was hypothesized that, in humans, these proteins contain conserved cytotoxic T-lymphocyte (CTL) target epitopes. Four HLA-restricted conserved epitopes of BKV, JCV and SV40 were identified: HLA-B*07, -B*08 and -DRB1*0901 for T-ag and -A*0201 for VP1. T cells cultured in vitro that were specific for one viral antigen recognized other conserved epitopes. CTLs generated from BKV T-ag and VP1 peptide were cytotoxic to DC targets pulsed with either BKV or JCV. Therefore, infection by one of the two viruses (BKV and JCV) could establish cross-immunity against the other. Although cross-cytotoxicity experiments were not performed with SV40, cross-recognition data from conserved antigen epitopes of polyomaviruses suggest strongly that cross-immunity might also exist among the three viruses. (literal)
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