Production and fingerprinting of virus-free clones in a reflowering globe artichoke (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Production and fingerprinting of virus-free clones in a reflowering globe artichoke (Articolo in rivista) (literal)

Anno

• 2010-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1007/s11240-009-9654-3 (literal)

Alternative label

• Acquadro A., Papanice M., Lanteri S., Bottalico G., Portis E., Campanale A., Finetti-Sialer M.M., Mascia T., Sumerano P. and Gallitelli D (2010)
  Production and fingerprinting of virus-free clones in a reflowering globe artichoke
  in Plant cell, tissue and organ culture (Dordr., Online)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Acquadro A., Papanice M., Lanteri S., Bottalico G., Portis E., Campanale A., Finetti-Sialer M.M., Mascia T., Sumerano P. and Gallitelli D (literal)

Pagina inizio

• 329 (literal)

Pagina fine

• 337 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 100 (literal)

Rivista

• Plant cell, tissue and organ culture (Rivista)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• A. Acquadro ? S. Lanteri ? E. Portis DIVAPRA Plant Genetics and Breeding, UNITO M. A. Papanice ? G. Bottalico ? M.M. Finetti-Sialer ? T.Mascia ? D. Gallitelli Dipartimento di Protezione delle Piante e Microbiologia Applicata, A. Campanale Istituto di Virologia Vegetale del CNR, Sezione di Bari P. Sumerano Consorzio di Difesa e Valorizzazione delle Produzioni Agricole della Provincia di Brindisi (literal)

Titolo

• Production and fingerprinting of virus-free clones in a reflowering globe artichoke (literal)

Abstract

• Most of the 24 viruses which infect globe artichoke are detrimental to the crop's performance and hamper the development of a nursery activity in the respect of current EU legislation. We describe a procedure to sanitize globe artichoke ''Brindisino'' from Artichoke Italian latent virus (AILV) and Artichoke latent virus (ArLV), while preserving its valuable early flowering trait. ArLV was successfully eliminated by meristem-tip culture,whileAILVwas removed when two rounds ofmeristem-tip culture were spaced outwith in vitro thermotherapy. In vivo thermotherapy, followed by meristem-tip culture, was also successful in producing virusfree material but was less efficient in terms of the number of plants recovered post treatment. Due to the multi-clonal composition of the populations at present in cultivation, the selected and sanitised clones were fingerprinted by applying microsatellite and AFLP (amplified fragment length polymorphism) markers.OneAFLP primer combination produced 28 informative fragments used to evaluate genetic relatedness among the clones in study. Our results demonstrates that AFLP-based molecular fingerprinting enables to verify the true to clone correspondence in nurseries, ensure the effective correspondence between the real and the declared identity of a clone, so that to avoid commercial frauds, and might represents a valuable tool for assessing somaclonal variation occuring during 'in vitro' propagation (literal)

Prodotto di

• Gestione e valorizzazione delle risorse genetiche vegetali con tecniche innovative (AG.P02.005.001) (Modulo)
• Istituto di Bioscienze e Biorisorse (IBBR) (Istituto)

Autore CNR

• MARIELLA MATILDE FINETTI SIALER (Unità di personale interno)

Incoming links:

Prodotto

• Istituto di Bioscienze e Biorisorse (IBBR) (Istituto)
• Gestione e valorizzazione delle risorse genetiche vegetali con tecniche innovative (AG.P02.005.001) (Modulo)

Autore CNR di

• MARIELLA MATILDE FINETTI SIALER (Unità di personale interno)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Plant cell, tissue and organ culture (Rivista)