Multiple effects of copper on NMDA receptor currents (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Multiple effects of copper on NMDA receptor currents (Articolo in rivista) (literal)

Anno

• 2014-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1016/j.brainres.2013.10.029 (literal)

Alternative label

• Marchetti, Carla; Baranowska-Bosiacka, Irena; Gavazzo, Paola (2014)
  Multiple effects of copper on NMDA receptor currents
  in Brain research; Elsevier, Amsterdam (Paesi Bassi)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Marchetti, Carla; Baranowska-Bosiacka, Irena; Gavazzo, Paola (literal)

Pagina inizio

• 20 (literal)

Pagina fine

• 31 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url

• http://www.sciencedirect.com/science/article/pii/S0006899313014200# (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 1542 (literal)

Rivista

• Brain research (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

• 12 (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Consiglio Nazionale delle Ricerche (CNR); Pomeranian Med Univ (literal)

Titolo

• Multiple effects of copper on NMDA receptor currents (literal)

Abstract

• Copper (Cu) is an essential metal present in the human brain and released from synaptic vesicles following neuronal depolarization. Cu is known to reduce the NMDA receptor (NR) current with IC50 approximate to 20 mu M. We have studied the effect of Cu on the NR current in cultured neonatal rat cerebellum granule cells (CGC) and in transiently transfected HEK293 cells (HEK), expressing either GluN1/GLUN2A or GluN1/GluN2B receptors. In CGCs, Cu causes a potentiation of the NR current at concentrations <30 mu M (EC50=4.6 mu M) and a block at higher concentrations (IC50=24 mu M). In Fura2 loaded CG.Cs, Cu (<= 30 mu M) caused an increase of NMDA-driven calcium influx. This facilitating effect was prevented by pre-treatment with the reducing agent DTT. Cu also caused an increase of the NR current in GluN1/GluN2A receptors (EC50=2 mu M) and a block at higher concentrations (IC50=26 mu M). Both facilitation and inhibition were independent of voltage. The effect of Cu was quantitatively similar in GluN1/G1uN2B receptors, which were potentiated by 10 mu M and inhibited by 100 mu M Cu. Potentiation was absent in mutants deleted of their entire amino terminal domain (ATD) of the protein, suggesting an involvement of this region in the interaction. These results indicate that Cu can facilitate the NR current at lower concentrations than those required for blocking it; this effect can have consequences on the activity of the metal at synaptic and extrasynaptic sites. (C) 2013 Elsevier B.V. All rights reserved. (literal)

Editore

• Elsevier (Editore)

Prodotto di

• Meccanismi molecolari della permeabilità di membrana (MD.P01.009.001) (Modulo)
• Istituto di biofisica (IBF) (Istituto)

Autore CNR

• PAOLA GAVAZZO (Unità di personale interno)
• CARLA MARCHETTI (Unità di personale interno)

Insieme di parole chiave

• Keywords of "Multiple effects of copper on NMDA receptor currents" (Insieme di parole chiave)

Incoming links:

Autore CNR di

• CARLA MARCHETTI (Unità di personale interno)
• PAOLA GAVAZZO (Unità di personale interno)

Prodotto

• Istituto di biofisica (IBF) (Istituto)
• Meccanismi molecolari della permeabilità di membrana (MD.P01.009.001) (Modulo)

Editore di

• Elsevier (Editore)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Brain research (Rivista)

Insieme di parole chiave di

• Keywords of "Multiple effects of copper on NMDA receptor currents" (Insieme di parole chiave)