http://www.cnr.it/ontology/cnr/individuo/prodotto/ID28217
Somatic embryogenesis and plant regeneration from pistil transverse thin cell layers of lemon (Citrus limon) (Articolo in rivista)
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- Somatic embryogenesis and plant regeneration from pistil transverse thin cell layers of lemon (Citrus limon) (Articolo in rivista) (literal)
- Anno
- 2008-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1080/11263500802150258 (literal)
- Alternative label
Sajeva M, Carra A, De Pasquale F, Carimi F (2008)
Somatic embryogenesis and plant regeneration from pistil transverse thin cell layers of lemon (Citrus limon)
in Plant Biosystems (Firenze, Testo stamp.); TAYLOR & FRANCIS LTD, 4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXON, ENGLAND, 4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXON, ENGLAND (Regno Unito)
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- Sajeva M, Carra A, De Pasquale F, Carimi F (literal)
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- http://www.tandfonline.com/doi/full/10.1080/11263500802150258#.UiwyEbxErcE (literal)
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- ISI Web of Science (WOS) (literal)
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- Istituto di Genetica Vegetale, Consiglio Nazionale delle Ricerche, Corso Calatafimi 414, 90129 Palermo, Italy; Dipartimento di Scienze Botaniche, Università di Palermo, 90123 Palermo, Italy (literal)
- Titolo
- Somatic embryogenesis and plant regeneration from pistil transverse thin cell layers of lemon (Citrus limon) (literal)
- Abstract
- Callus induction, somatic embryogenesis and plant regeneration were obtained in Citrus limon (L.) Burm. (cv. Femminello) from cultures of pistil transverse thin cell layer explants [(t)TCLs]. Explants were cultured on two different media, based on Murashige and Skoog salts and vitamins, supplemented with 500mg l-1 malt extract (MSI), or 500mg l-1 malt extract and 13.3 M 6-benzylaminopurine (MSII). Sucrose (146 mM) was used as carbon source. Somatic embryos appeared 3 months after culture initiation from stigma and style (t)TCLs; they were observed at the surface of the (t)TCL-derived callus. Although ovary (t)TCLs showed the highest callus formation, they never differentiated somatic embryos. Percentages of embryo formation from (t)TCLs incubated on MSI (13% and 2% for stigma and style, respectively) were lower than those from (t)TCLs incubated on MSII (36% and 7% for stigma and style, respectively). The embryogenic response of stigma (t)TCLs was usually higher than that of style (t)TCLs. After about 3 months, somatic embryos developed into plantlets at high frequencies (57% and 62% for stigma- and style-derived somatic embryos, respectively). (literal)
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