http://www.cnr.it/ontology/cnr/individuo/prodotto/ID281889
Release kinetics of gold nanoparticles from collagen microcapsules by total reflection X-ray fluorescence (Articolo in rivista)
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- Release kinetics of gold nanoparticles from collagen microcapsules by total reflection X-ray fluorescence (Articolo in rivista) (literal)
- Anno
- 2013-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1016/j.colsurfa.2012.11.012 (literal)
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Erokhina, Svetlana; Konovalov, Oleg; Bianchini, Paolo; Diaspro, Alberto; Ruggiero, Carmelina; Erokhin, Victor; Pastorino, Laura (2013)
Release kinetics of gold nanoparticles from collagen microcapsules by total reflection X-ray fluorescence
in Colloids and surfaces. A, Physicochemical and engineering aspects (Print); ELSEVIER SCIENCE BV, PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS, AMSTERDAM (Paesi Bassi)
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- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Erokhina, Svetlana; Konovalov, Oleg; Bianchini, Paolo; Diaspro, Alberto; Ruggiero, Carmelina; Erokhin, Victor; Pastorino, Laura (literal)
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- http://www.sciencedirect.com/science/article/pii/S0927775712007650# (literal)
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- a Department of Biochemistry and Molecular Biology, University of Parma, Viale Usberti 23 A, 43100 Parma, Italy; b European Synchrotron Radiation Facility, 38043 Grenoble Cedex, France; c Nanophysics, Fondazione Istituto Italiano di Tecnologia, 16163 Genoa, Italy; d Department of Informatics, Bioengineering, Robotics and Systems Engineering, University of Genova, Via all'Opera Pia 13, 16145 Genova, Italy; e CNR-IMEM, and Department of Physics, University of Parma, Viale Usberti 7A, 43124 Parma, Italy (literal)
- Titolo
- Release kinetics of gold nanoparticles from collagen microcapsules by total reflection X-ray fluorescence (literal)
- Abstract
- One of the main challenges in nanotechnology based drug delivery systems is the design of novel materials for patient specific therapeutic efficacy. This can be achieved taking into account changes in the expression profiles of related proteins. Here, we focus on collagen type I micro-capsules for releasing molecules using a biological stimulus elicited by over-expressed collagenolytic matrix metalloproteinase I (MMPI). We have carried out label-free real time monitoring of the release kinetics of gold nanoparticles induced by MMPI in order to define the pore opening process by synchrotron radiation based total reflection X-ray fluorescence. Scanning electron and confocal laser scanning microcopy investigations have been carried out in order to further characterize the interaction between collagen micro-capsules and MMP1. The results enable to predict and to engineer the release time for drug molecules of different sizes. Moreover, the proposed label-free approach can be adopted for the visualization of pore formation and of release kinetics for nano-engineered polymeric capsules. (C) 2012 Elsevier B.V. All rights reserved. (literal)
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