Enhancement of plasmid-mediated transgene expression. (Contributo in volume (capitolo o saggio))

Type
Label
  • Enhancement of plasmid-mediated transgene expression. (Contributo in volume (capitolo o saggio)) (literal)
Anno
  • 2014-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1007/978-1-4939-0410-5_2 (literal)
Alternative label
  • Fioretti, Daniela; Iurescia, Sandra; Rinaldi, Monica (2014)
    Enhancement of plasmid-mediated transgene expression.
    in DNA vaccines- Methods and Protocols Third Edition, 2014
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Fioretti, Daniela; Iurescia, Sandra; Rinaldi, Monica (literal)
Pagina inizio
  • 11 (literal)
Pagina fine
  • 20 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#titoloVolume
  • DNA vaccines- Methods and Protocols Third Edition (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#volumeInCollana
  • 1143 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali
  • 10 (literal)
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Institute of Translational Pharmacology (IFT), National Research Council (CNR), via Fosso del Cavaliere 100, 00133, Rome, Italy. (literal)
Titolo
  • Enhancement of plasmid-mediated transgene expression. (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#curatoriVolume
  • Rinaldi, Monica; Fioretti, Daniela; Iurescia, Sandra (literal)
Abstract
  • A large number of studies aimed at the treatment of cancer, autoimmune and metabolic diseases, neurodegenerative disorders, allergic diseases, as well as muscle disorders strengthen the fact that gene therapy could represent an alternative method to treat human diseases where conventional approaches are less effective. To improve transgene expression from plasmid vectors, DNA nuclear targeting sequences (DTSs) can be introduced in a vector backbone to increase in vivo expression up to 20-fold using electroporation (EP) delivery in muscle tissue. The purpose of this chapter is to represent a step-by-step strategy for the construction of a plasmid vector with enhanced efficiency of nuclear plasmid uptake and the methodic for the in vivo efficiency evaluation of the obtained expression vector. (literal)
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