Molecular characterization of b-amyrin-like synthase from Aster sedifolius L. and triterpenoid saponin analysis (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Molecular characterization of b-amyrin-like synthase from Aster sedifolius L. and triterpenoid saponin analysis (Articolo in rivista) (literal)

Anno

• 2008-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1016/j.plantsci.2008.04.004 (literal)

Alternative label

• Cammareri M.; Consiglio F.; Pecchia P.; Corea G.; Lanzotti V.; Ibeas J.I.; Tava A.; Conicella C. (2008)
  Molecular characterization of b-amyrin-like synthase from Aster sedifolius L. and triterpenoid saponin analysis
  in Plant science (Limerick)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Cammareri M.; Consiglio F.; Pecchia P.; Corea G.; Lanzotti V.; Ibeas J.I.; Tava A.; Conicella C. (literal)

Pagina inizio

• 255 (literal)

Pagina fine

• 261 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 175 (literal)

Rivista

• Plant science (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

• 7 (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• CNR-IGV, Research Institute of Plant Genetics, Research Division: Portici, Via Universita` 133, 80055 Portici, Italy Department of Chemistry of Natural Products, University of Naples Federico II, Via D. Montesano 49, 80131 Naples, Italy DISTAAM, University of Molise, Via De Sanctis, 86100 Campobasso, Italy Centro Andaluz de Bilog´a del Desarrollo, UPO-CSIC, Carretera de Utrera Km 1, 41013 Sevilla, Spain C.R.A.-Istituto Sperimentale per le Colture Foraggere, viale Piacenza 29, 26900 Lodi, Italy (literal)

Titolo

• Molecular characterization of b-amyrin-like synthase from Aster sedifolius L. and triterpenoid saponin analysis (literal)

Abstract

• Triterpenoid saponins are secondary metabolites showing a remarkable structural variety, as well as notable biological activities. They are synthesised via the isoprenoid pathway by 2,3-oxidosqualene cyclization mediated by oxidosqualene cyclase (OSC) enzymes. In our previous work, three new oleanane-type triterpene saponins (astersedifolioside A-C) were isolated from the aerial tissues of Aster sedifolius L. In this work, the full-length cDNA of a new OSC, designated AsOXA1, was isolated from A. sedifolius. The AsOXA1 open reading frame consisting of 2286 bp was predicted to encode a protein of 761 amino acid residues. The deduced amino acid sequence of AsOXA1 showed QW and DCTAE motifs which are highly conserved among the known triterpene synthases. In addition, the comparative and phylogenetic analysis evidenced that AsOXA1 was closely related to other plant OSCs, and particularly, bamyrin synthases including that one of Panax ginseng with which it shared a high degree of identity (79%). Recombinant AsOXA1 has been shown to produce b-amyrin in yeast. RT-PCR analysis evidenced that AsOXA1 was a tissue-specific gene differentially expressed during plant development. Transcripts of AsOXA1 accumulated strongly in leaf at the early reproductive stage but were deficient in root. On the contrary, the accumulation of astersedifoliosides resulted 10-fold higher in roots than in aerial tissues. Molecular and biochemical results have been discussed. (literal)

Prodotto di

• Ottenimento di nuovi prodotti per la salute, l'alimentazione e l'industria mediante interventi genetici e biotecnologici nelle piante (AG.P01.006.001) (Modulo)
• Istituto di Bioscienze e Biorisorse (IBBR) (Istituto)

Autore CNR

• MARIA FEDERICA CONSIGLIO (Unità di personale interno)
• CLARA CONICELLA (Unità di personale interno)
• MARIA CAMMARERI (Unità di personale interno)

Insieme di parole chiave

• Parole chiave di "Molecular characterization of b-amyrin-like synthase from Aster sedifolius L. and triterpenoid saponin analysis" (Insieme di parole chiave)

Incoming links:

Prodotto

• Istituto di Bioscienze e Biorisorse (IBBR) (Istituto)
• Ottenimento di nuovi prodotti per la salute, l'alimentazione e l'industria mediante interventi genetici e biotecnologici nelle piante (AG.P01.006.001) (Modulo)

Autore CNR di

• MARIA FEDERICA CONSIGLIO (Unità di personale interno)
• MARIA CAMMARERI (Unità di personale interno)
• CLARA CONICELLA (Unità di personale interno)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Plant science (Rivista)

Insieme di parole chiave di

• Parole chiave di "Molecular characterization of b-amyrin-like synthase from Aster sedifolius L. and triterpenoid saponin analysis" (Insieme di parole chiave)