http://www.cnr.it/ontology/cnr/individuo/prodotto/ID28072

Salicylic acid activates nitric oxide synthesis in Arabidopsis (Articolo in rivista)

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Salicylic acid activates nitric oxide synthesis in Arabidopsis (Articolo in rivista) (literal)

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Zottini M, Costa A, De Michele R, Ruzzene M, Carimi F, Lo Schiavo F (2007)
Salicylic acid activates nitric oxide synthesis in Arabidopsis
in Journal of experimental botany; Oxford University Press, Oxford (Regno Unito)
(literal)

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Dipartimento di Biologia, Università Degli Studi di Padova, Via U. Bassi 58/B, I-35131 Padova, Italy; Istituto di Genetica Vegetale, Palermo (CNR), Corso Calatafimi 414, I-90128 Palermo, Italy (literal)

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Abstract

The relationship between nitric oxide (NO) and salicylic acid (SA) was investigated in Arabidopsis thaliana. Here it is shown that SA is able to induce NO synthesis in a dose-dependent manner in Arabidopsis. NO production was detected by confocal microscopic analysis and spectrofluorometric assay in plant roots and cultured cells. To identify the metabolic pathways involved in SA-induced NO synthesis, genetic and pharmacological approaches were adopted. The analysis of the nia1,nia2 mutant showed that nitrate reductase activity was not required for SA-induced NO production. Experiments performed in the presence of a nitric oxide synthase (NOS) inhibitor suggested the involvement of NOS-like enzyme activity in this metabolic pathway. Moreover, the production of NO by SA treatment of Atnos1 mutant plants was strongly reduced compared with wild-type plants. Components of the A signalling pathway giving rise to NO production were identified, and both calcium and casein kinase 2 (CK2) were demonstrated to be involved. Taken together, these results suggest that SA induces NO production at least in part through the activity of a NOS-like enzyme and that calcium and CK2 activity are essential components of the signalling cascade. (literal)

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