Human second-trimester amniotic fluid cells are able to create embryoid body-like structures \"in vitro\" and to show typical expression profiles of embryonic and primordial germ cells. (Articolo in rivista)

Type
Label
  • Human second-trimester amniotic fluid cells are able to create embryoid body-like structures \"in vitro\" and to show typical expression profiles of embryonic and primordial germ cells. (Articolo in rivista) (literal)
Anno
  • 2014-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.3727/096368914X678553 (literal)
Alternative label
  • Antonucci I, Di Pietro R, Alfonsi M, Centurione MA, Centurione L, Sancilio S, Pelagatti F, D'Amico MA, Di Baldassarre A, Piattelli A, Tetè S, Palka G, Borlongan CV, Stuppia L. (2014)
    Human second-trimester amniotic fluid cells are able to create embryoid body-like structures "in vitro" and to show typical expression profiles of embryonic and primordial germ cells.
    in Cell transplantation (Online)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Antonucci I, Di Pietro R, Alfonsi M, Centurione MA, Centurione L, Sancilio S, Pelagatti F, D'Amico MA, Di Baldassarre A, Piattelli A, Tetè S, Palka G, Borlongan CV, Stuppia L. (literal)
Pagina inizio
  • 1501 (literal)
Pagina fine
  • 1515 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 23 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
  • 12 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Antonucci, Ivana; G. d'Annunzio University, Psychological, Humanities and Territorial Sciences; Stem Tech Group, Center for Aging Studies (CESI), Di Pietro, Roberta; G. D'Annunzio University, Medicine and Aging Sciences; Stem Tech Group, Center for Aging Studies (CESI), Alfonsi, Melissa; G. d'Annunzio University, Clinical, Oral and Biotechnological Sciences Centurione, Maria; G. D'Annunzio University, Medicine and Aging Sciences; Stem Tech Group, Center for Aging Studies (CESI), Centurione, Lucia; G. D'Annunzio University, Medicine and Aging Sciences; Stem Tech Group, Center for Aging Studies (CESI), Sancilio, Silvia; G. D'Annunzio University, Medicine and Aging Sciences Marroncelli, Valentina; G. d'Annunzio University, Psychological, Humanities and Territorial Sciences; Stem Tech Group, Center for Aging Studies (CESI), Pelagatti, Francesca; G. d'Annunzio University, Psychological, Humanities and Territorial Sciences; Stem Tech Group, Center for Aging Studies (CESI), D'Amico, Maria; G. D'Annunzio University, Medicine and Aging Sciences Di Baldassare, Angela; G. D'Annunzio University, Medicine and Aging Sciences Piattelli, Adriano; G. d'Annunzio University, Clinical, Oral and Biotechnological Sciences Tetè, Stefano; G. d'Annunzio University, Clinical, Oral and Biotechnological Sciences Palka, Giandomenico; G. d'Annunzio University, Clinical, Oral and Biotechnological Sciences Stuppia, Liborio; G. d'Annunzio University, Psychological, Humanities and Territorial Sciences ; Stem Tech Group, Center for Aging Studies (CESI), (literal)
Titolo
  • Human second-trimester amniotic fluid cells are able to create embryoid body-like structures \"in vitro\" and to show typical expression profiles of embryonic and primordial germ cells. (literal)
Abstract
  • Human Amniotic Fluid-derived Stem Cells (AFSCs) represent a novel class of broadly multipotent stem cells sharing characteristics of both embryonic and adult stem cells. However, both the origin of these cells and their actual properties in terms of pluripotent differentiation potential are still debated. In order to verify the presence of features of pluripotency in human second-trimester AFSCs, we have investigated the ability of these cells to form in vitro three-dimensional aggregates, known as embryoid bodies (EBs), and to express specific genes of embryonic stem cells (ESCs) and primordial germ cells (PGCs). EBs were obtained after 5 days of AFSCs culture in suspension, and showed positivity for alkaline phosphatase (AP) staining and for specific markers of pluripotency (OCT4 and SOX2). Moreover, EB derived cells showed the expression of specific transcripts of the three germ layers. RT-PCR analysis, carried out at different culture times (2nd, 3th, 4th, 5th and 8th passage), evidenced the presence of specific markers of ESCs (such as FGF4 and DAPPA4), as well as of markers typical of PGCs, and in particular genes involved in early stages of germ cell development (Fragilis, Stella, Vasa, c-Kit, Rnf17). Finally, the expression of genes related to the control of DNA methylation (DNMT3A, DNMT3b1, DNMT1, DNMT3L, MBD1, MBD2, MBD3, MDB4, MeCP2) as well as the lack of inactivation of the X-chromosome in female samples was also evidenced. Taken together, these data provide further evidence for the presence of common features among human AFSCs, PGC and ESCs. (literal)
Prodotto di
Autore CNR

Incoming links:


Prodotto
Autore CNR di
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi
data.CNR.it