http://www.cnr.it/ontology/cnr/individuo/prodotto/ID28023

Growth and senescence of Medicago truncatula cultured cells are associated with characteristic mitochondrial morphology (Articolo in rivista)

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Growth and senescence of Medicago truncatula cultured cells are associated with characteristic mitochondrial morphology (Articolo in rivista) (literal)

Anno

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Alternative label

Zottini M, Barizza E, Bastianelli F, Carimi F, Lo Schiavo F (2006)
Growth and senescence of Medicago truncatula cultured cells are associated with characteristic mitochondrial morphology
in New phytologist (Print); Blackwell Publishing, Oxford (Regno Unito)
(literal)

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http://onlinelibrary.wiley.com/doi/10.1111/j.1469-8137.2006.01830.x/pdf (literal)

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Istituto di Genetica Vegetale, Consiglio Nazionale delle Ricerche, Corso Calatafimi 414, 90129 Palermo, Italy; Dipartimento di Biologia, Università Degli Studi di Padova, Via G. Colombo 3, Padua I-35121, Italy (literal)

Titolo

Growth and senescence of Medicago truncatula cultured cells are associated with characteristic mitochondrial morphology (literal)

Abstract

Here mitochondrial morphology and dynamics were investigated in Medicago truncatula cell-suspension cultures during growth and senescence. Cell biology techniques were used to measure cell growth and death in culture. Mitochondrial morphology was investigated in vivo using a membrane potential sensor probe coupled with confocal microscopy. 2) Expression of a senescence-associated gene (MtSAG) was evaluated in different cell-growth phases. Mitochondria appeared as numerous, punctuate organelles in cells at the beginning of the subculture cycle, while interconnected networks were observed in actively growing cells. In senescent cells, giant mitochondria were associated with dying cells. The release of cytochrome c from mitochondria was detected in different growth phases of cultured cells. 3) Studies on plant cell cultures allowed us to identify physiological and molecular markers of senescence and cell death, and to associate distinct itochondrial morphology with cells under different physiological conditions. (literal)

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