Glomerular Autoimmune Multicomponents of Human Lupus Nephritis In Vivo: alpha-Enolase and Annexin AI. (Articolo in rivista)

Type
Label
  • Glomerular Autoimmune Multicomponents of Human Lupus Nephritis In Vivo: alpha-Enolase and Annexin AI. (Articolo in rivista) (literal)
Anno
  • 2014-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1681/ASN.2013090987 (literal)
Alternative label
  • Bruschi M, Sinico RA, Moroni G, Pratesi F, Migliorini P, Galetti M, Murtas C, Tincani A, Madaio M, Radice A, Franceschini F, Trezzi B, Bianchi L, Giallongo A, Gatti R, Tardanico R, Scaloni A, D'Ambrosio C, Carnevali ML, Messa P, Ravani P, Barbano G, Bianco B, Bonanni A, Scolari F, Martini A, Candiano G, Allegri L, Ghiggeri GM. (2014)
    Glomerular Autoimmune Multicomponents of Human Lupus Nephritis In Vivo: alpha-Enolase and Annexin AI.
    in Journal of the American Society of Nephrology
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Bruschi M, Sinico RA, Moroni G, Pratesi F, Migliorini P, Galetti M, Murtas C, Tincani A, Madaio M, Radice A, Franceschini F, Trezzi B, Bianchi L, Giallongo A, Gatti R, Tardanico R, Scaloni A, D'Ambrosio C, Carnevali ML, Messa P, Ravani P, Barbano G, Bianco B, Bonanni A, Scolari F, Martini A, Candiano G, Allegri L, Ghiggeri GM. (literal)
Pagina inizio
  • 2483 (literal)
Pagina fine
  • 2498 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url
  • http://jasn.asnjournals.org/content/early/2014/04/30/ASN.2013090987.abstract (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 25 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali
  • 16 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
  • 11 (literal)
Note
  • PubMe (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Laboratory on Pathophysiology of Uremia, Divisions of Nephrology, Dialysis, and Transplantation, and Paediatric Rheumatology, Istituto Giannina Gaslini, Genoa, Italy; Division of Nephrology and Section of Clinical Immunology, San Carlo Hospital, Milan, Italy; Division of Nephrology and Dialysis, Scientific Institute for Research and Health Care (IRCCS) Fondazione Ospedale Maggiore, Mangiagalli, Regina Elena, Milan, Italy; Clinical Immunology Unit, Department of Internal Medicine, University of Pisa, Pisa, Italy; Departments of ?Clinical and Experimental Medicine, Biomedical, Biotechnological and Translational Sciences, and Italian Workers' Compensation Authority (INAIL) Research Center, University of Parma, Parma, Italy; Rheumatology and Clinical Immunology, Spedali Civili and University of Brescia, Brescia, Italy; Department of Medicine, Georgia Health Sciences University, Augusta, Georgia; Institute of Biomedicine and Molecular Immunology, CNR, Palermo, Italy; Service of Pathological Anatomy, Division of Nephrology, Spedali Civili di Brescia, Brescia, Italy; CNR, Institute for Animal Production System in Mediterranean Environment (ISPAAM), Naples, Italy; Division of Nephrology, University of Calgary, Calgary, Alberta, Canada; and Division of Nephrology, University of Brescia, Montichiari Hospital, Brescia, Italy (literal)
Titolo
  • Glomerular Autoimmune Multicomponents of Human Lupus Nephritis In Vivo: alpha-Enolase and Annexin AI. (literal)
Abstract
  • Renal targets of autoimmunity in human lupus nephritis (LN) are unknown. We sought to identify autoantibodies and glomerular target antigens in renal biopsy samples from patients with LN and determine whether the same autoantibodies can be detected in circulation. Glomeruli were microdissected from biopsy samples of 20 patients with LN and characterized by proteomic techniques. Serum samples from large cohorts of patients with systemic lupus erythematosus (SLE) with and without LN and other glomerulonephritides were tested. Glomerular IgGs recognized 11 podocyte antigens, with reactivity varying by LN pathology. Notably, IgG2 autoantibodies against ?-enolase and annexin AI were detected in 11 and 10 of the biopsy samples, respectively, and predominated over other autoantibodies. Immunohistochemistry revealed colocalization of ?-enolase or annexin AI with IgG2 in glomeruli. High levels of serum anti-?-enolase (>15 mg/L) IgG2 and/or anti-annexin AI (>2.7 mg/L) IgG2 were detected in most patients with LN but not patients with other glomerulonephritides, and they identified two cohorts: patients with high anti-?-enolase/low anti-annexin AI IgG2 and patients with low anti-?-enolase/high anti-annexin AI IgG2. Serum levels of both autoantibodies decreased significantly after 12 months of therapy for LN. Anti-?-enolase IgG2 recognized specific epitopes of ?-enolase and did not cross-react with dsDNA. Furthermore, nephritogenic monoclonal IgG2 (clone H147) derived from lupus-prone MRL-lpr/lpr mice recognized human ?-enolase, suggesting homology between animal models and human LN. These data show a multiantibody composition in LN, where IgG2 autoantibodies against ?-enolase and annexin AI predominate in the glomerulus and can be detected in serum. (literal)
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