Resistance in wild olive against the defoliating Verticillium dahliae pathotype (Abstract/Comunicazione in atti di convegno)

Type
Label
  • Resistance in wild olive against the defoliating Verticillium dahliae pathotype (Abstract/Comunicazione in atti di convegno) (literal)
Anno
  • 2013-01-01T00:00:00+01:00 (literal)
Alternative label
  • Jiménez-Fernández D., Trapero-Casas J.L., Gramaje D., Landa B.B., Navas-Cortés J.A., Bubici, G., Cirulli M., Jiménez-Díaz R. M. (2013)
    Resistance in wild olive against the defoliating Verticillium dahliae pathotype
    in 11th International Verticillium Symposium, Georg-August-Universität,Göttingen, Germany, 5-8 maggio 2013
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Jiménez-Fernández D., Trapero-Casas J.L., Gramaje D., Landa B.B., Navas-Cortés J.A., Bubici, G., Cirulli M., Jiménez-Díaz R. M. (literal)
Pagina inizio
  • 45 (literal)
Pagina fine
  • 45 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url
  • http://dpg.phytomedizin.org/fileadmin/daten/04_Verlag/02_SP/10_Vert2013/0294-sp-2013-Vert-0.pdf (literal)
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  • 1 (literal)
Note
  • Comunicazione (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • JFD, JDRM: College of Agriculture and Forestry, University of Córdoba, Campus de Excelencia Internacional Agroalimentario, Córdoba, Spain TCJL, GD, LBB, NCJA, JDRM: Institute for Sustainable Agriculture, Spanish National Research Council (CSIC), Córdoba, Spain BG: Istituto di Virologia Vegetale, UOS Bari, CNR, Bari, Italy CM: Dipartimento di Scienze del Suolo della Pianta e degli Alimenti, Università degli Studi di Bari 'Aldo Moro', Bari, Italy (literal)
Titolo
  • Resistance in wild olive against the defoliating Verticillium dahliae pathotype (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#isbn
  • 978-3-941261-12-9 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#curatoriVolume
  • BIRGER KOOPMANN, ANDREAS VON TIEDEMANN (literal)
Abstract
  • Rootstocks highly resistant to the highly virulent Verticillium dahliae defoliating (D) pathotype would be of much interest for the management of Verticillium wilt in olive and growing of Verticillium-susceptible olive cultivars in geographic areas where D V. dahliae prevails. Recently, research done at the University of Bari, Italy, and University of Córdoba, Spain, have led to the identification of some wild olive genotypes that could be of use as resistant rootstocks, including the currently patented clones STOPVERT and OUTVERT, AC13 and AC18. To further characterize the resistance reaction shown by those genotypes in previous studies, we have carried out a series of experiments using standardized protocols and controlled conditions optimal for development of Verticillium wilt. Own-rooted plants of a range of ages were inoculated with a range of high inoculum concentrations of selected, highly virulent D isolate V138I by root dipping and/or transplanting in an artificially infested soil mixture. Plants were inoculated once or twice in a sequence, and incubated in the growth chamber for 3 to 4 months under optimal conditions for disease development. Disease reaction was assessed by the development of foliar symptoms, isolation of the pathogen from the lower, middle, and upper main stem, and molecular quantification of the fungus in the sampled tissues using a real-time quantitative PCR (qPCR) protocol with a detection limit of 18 fg of V. dahliae DNA in infected, symptomless tissues. Clones STOPVERT and OUTVERT showed a symptomless reaction to inoculation compared with 100 % dead plants in susceptible 'Picual' olive and mild disease reaction in tolerant 'Frantoio'. V. dahliae was isolated from middle stem parts of STOPVERT and OUTVERT plants to a lesser extent than from the lower stem, but isolations from 'Frantoio' plants yielded the fungus from all stem parts at similar proportions. On average, the concentration of V. dahliae DNA per 100 ng of stem DNA ranged from 5.6 to 41.1 pg in STOPVERT plants, from 13.7 to 80.9 pg in OUTVERT plants, and from 94.6 to 141.6 pg in 'Frantoio' plants. The larger of those concentrations is 120 times lower than that found in susceptible 'Picual' olive. Extending the time of incubation of infected STOPVERT and OUTVERT plants reduced the frequency of successful isolations and quantification from previously infected tissues. Clones AC13 and AC18 also showed a highly resistant reaction to root-dip inoculation with V. dahliae 138I, though slight symptoms developed in some 'AC18' plants. Average V. dahliae DNA concentration per 100 ng of stem DNA was 10,9 and 86,7 pg in 'AC13' and 'AC18' plants, respectively, compared with 42,9 pg and 16,6 ng in 'Frantoio' and 'Picual' olives. Histopathological assessment of the plants reaction is in progress. Also, experiments are being conducted to determine the influence of genetic and geographic diversity of D V. dahliae isolates on the resistant reaction of the wild olive clones. Research supported by grants P10-AGR 6082 from CICE-Junta de Andalucía, Spain and the 'Organización Interprofesional del Aceite de Oliva Español-CITOLIVA'. (literal)
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