http://www.cnr.it/ontology/cnr/individuo/prodotto/ID27970
Protein patterns, characterized by computer image analysis, of lentil embryo axes germinatine under salt stress (Articolo in rivista)
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- Protein patterns, characterized by computer image analysis, of lentil embryo axes germinatine under salt stress (Articolo in rivista) (literal)
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- 2004-01-01T00:00:00+01:00 (literal)
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- ISI Web of Science (WOS) (literal)
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- Titolo
- Protein patterns, characterized by computer image analysis, of lentil embryo axes germinatine under salt stress (literal)
- Abstract
- Following 16, 40 and 64 h exposure to 0.33 M NaCl given after 8 h water imbibition, lentil seeds showed a gradual decrease of germination upon their transfer to water. These salt related changes were accompanied by modifications in the protein patterns of embryo axes as revealed by two-dimensional electrophoresis separation and by the computer image analysis of protein spots. In comparison with 8 h water imbibed seeds, prominent proteins comprised between the 5.1 7.6 pH isoelectric point in the first dimension and 75 - 50 kDa molecular mass in the second dimension showed a significant increase in their abundance as salt exposure increased. On transfer to water to complete germination, the content of many of these proteins decreased at 24 h in 2 - 3 cm length embryo axes in comparison with the corresponding embryo axes of seeds continuously imbibed in water for 24 h. Some groups of proteins ranging between 15.5-17.3 kDa, already present after 8 h water imbibition, were not detectable after 24 h but were expressed in seeds exposed to NaCl and transferred to water for 24 h. Up- and down- regulated proteins in lentil embryo axes, imbibed under non-lethal salt stress conditions, have been tentatively identified by comparison with the protein map of germinating seeds of the model plant Arabidopsis. (literal)
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