Fluorescent Recovery after Photobleaching (FRAP) Analysis of Nuclear Export Rates Identifies Intrinsic Features of Nucleocytoplasmic Transport (Articolo in rivista)

Type
Label
  • Fluorescent Recovery after Photobleaching (FRAP) Analysis of Nuclear Export Rates Identifies Intrinsic Features of Nucleocytoplasmic Transport (Articolo in rivista) (literal)
Anno
  • 2012-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1074/jbc.M111.304899 (literal)
Alternative label
  • F. Cardarelli [ 1 ] ; L. Tosti [ 2,3 ] ; Serresi M. [ 1 ] ; F. Beltram [ 1,2,3 ] ; R. Bizzarri [ 1,2,3 ] (2012)
    Fluorescent Recovery after Photobleaching (FRAP) Analysis of Nuclear Export Rates Identifies Intrinsic Features of Nucleocytoplasmic Transport
    in The Journal of biological chemistry (Print); AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC, BETHESDA (Stati Uniti d'America)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • F. Cardarelli [ 1 ] ; L. Tosti [ 2,3 ] ; Serresi M. [ 1 ] ; F. Beltram [ 1,2,3 ] ; R. Bizzarri [ 1,2,3 ] (literal)
Pagina inizio
  • 5554 (literal)
Pagina fine
  • 5561 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 287 (literal)
Rivista
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • [ 1 ] Ist Italiano Tecnol, Ctr Nanotechnol Innovat NEST, I-56124 Pisa, Italy [ 2 ] Scuola Normale Super Pisa, NEST, I-56124 Pisa, Italy [ 3 ] Ist Nanosci CNR, I-56124 Pisa, Italy (literal)
Titolo
  • Fluorescent Recovery after Photobleaching (FRAP) Analysis of Nuclear Export Rates Identifies Intrinsic Features of Nucleocytoplasmic Transport (literal)
Abstract
  • A quantitative description of carrier-mediated nuclear export in live cells is presented. To this end, we fused a prototypical leucine-rich nuclear export signal (NES) to GFP as a cargo model and expressed the fluorescent chimera in live CHO-K1 cells. By modeling FRAP data, we calculate the NES affinity for the export machinery and the maximum rate of nuclear export achievable at saturation of endogenous carriers. The measured active-export time through the Nuclear Pore Complex (NPC) is 18 ms, remarkably similar to the previously determined active-import rate. Also, our results reveal that active export/import and active export/passive diffusion fluxes are uncoupled, thus complementing previous reports on active import/passive diffusion uncoupling. These findings suggest differential gating at the NPC level. (literal)
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