Flow Cytometry, SSR and modified AFLP markers for the identification of zygotic plantlets in backcrosses between ?Femminello? lemon cybrids (2n and 4n) and a diploid clone of ?Femminello? lemon (Citrus limon L. Burm. F.) tolerant to mal secco disease (Articolo in rivista)

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  • Flow Cytometry, SSR and modified AFLP markers for the identification of zygotic plantlets in backcrosses between ?Femminello? lemon cybrids (2n and 4n) and a diploid clone of ?Femminello? lemon (Citrus limon L. Burm. F.) tolerant to mal secco disease (Articolo in rivista) (literal)
Anno
  • 2003-01-01T00:00:00+01:00 (literal)
Alternative label
  • Scarano M.-T. 1, Tusa N. 2, Abbate L. 3, Lucretti S. 4, Nardi L. 5, Ferrante S. 6, (2003)
    Flow Cytometry, SSR and modified AFLP markers for the identification of zygotic plantlets in backcrosses between ?Femminello? lemon cybrids (2n and 4n) and a diploid clone of ?Femminello? lemon (Citrus limon L. Burm. F.) tolerant to mal secco disease
    in Plant science (Limerick)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Scarano M.-T. 1, Tusa N. 2, Abbate L. 3, Lucretti S. 4, Nardi L. 5, Ferrante S. 6, (literal)
Pagina inizio
  • 1009 (literal)
Pagina fine
  • 1017 (literal)
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  • Sono state utilizzate le tecniche di analisi molecolari denominare AFLP modificata e SSR. In particolare la tecnica AFLP modificata è stata testata per la prima volta per la caratterizzazione molecolare di ibridi zigotici di agrumi. I risultati ottenuti hanno comunque messo in evidenza la maggiore efficata dell'analisi dei microsatelliti, rispetto all'analisi AFLP, per la determinazione degli ibridi zigotici ottenuti. http://scienceserver.cilea.it/cgi-bin/sciserv.pl?collection=journals&journal=01689452&issue=v164i0006&article=1009_fcsamafttmsd Impact factor della rivista = 1.556 (literal)
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  • 164 (literal)
Rivista
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  • IF 2003: 1.652 (literal)
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  • Articolo su rivista internazionale riguardante la caratterizzazione molecolare di ibridi zigotici ottenuti da incroci di ritorno tra cibridi diploidi e tetraploidi di limone con il limone cv \"Femminello\". (literal)
Note
  • ISI Web of Science (WOS) (literal)
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  • 1 CNR, 2 CNR, 3 CNR, 4 ENEA, 5 ENEA, 6 CNR (literal)
Titolo
  • Flow Cytometry, SSR and modified AFLP markers for the identification of zygotic plantlets in backcrosses between ?Femminello? lemon cybrids (2n and 4n) and a diploid clone of ?Femminello? lemon (Citrus limon L. Burm. F.) tolerant to mal secco disease (literal)
Abstract
  • One of the most important goals in genetic improvement of lemon in the Mediterranean area is certainly the obtainment of new genotypes tolerant or resistant to mal secco disease. Two lemon cybrids (one diploid and one autotetraploid) with an intermediate degree of resistance to mal secco disease, spontaneously obtained by symmetric protoplast fusion between ‘Valencia’ orange and ‘Femminello’ lemon, were used as mother plants in backcrosses with a diploid clone of ‘Femminello’ Lemon Tolerant to the Mal Secco disease (LTMS) to improve tolerance and fruit quality. Since ‘Femminello’ lemon normally reproduces apomictically by nucellar embryony, an embryo-rescue technique was applied 105 days after pollination to recover zygotic embryos. A total of 66 plantlets were regenerated as follows: 20 from the 4nX2n cross, and 46 from the 2nX2n cross. In order to distinguish zygotic embryos from nucellars, flow cytometry was applied on all the 66 plantlets; and a modified AFLP (Amplified Fragment-Length Polymorphism) technique and microsatellite analysis were carried out on a sample of 20 seedlings (6 triploids and 14 diploids). Here we report the usefulness and reliability of the tested techniques and discuss the results obtained. Flow cytometry (FCM) was useful only in inter-ploid crosses, identifying 6 triploids, 4 of which were also detected by SSR (Simple Sequence Repeat) analysis and only 2 by modified AFLP. Among the 14 tested diploids, 5 zygotic genotypes were identified by SSR and 4 by modified AFLP. In our hands, the SSR technique appeared to be more suitable than modified AFLP. (literal)
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