http://www.cnr.it/ontology/cnr/individuo/prodotto/ID27937

Characterization of high and low-molecular weight glutenin subunits associated to the D genome of Aegilops tauschii in a collection of synthetic hexaploid wheats (Articolo in rivista)

Type

Articolo in rivista (Classe)
Prodotto della ricerca (Classe)

Label

Characterization of high and low-molecular weight glutenin subunits associated to the D genome of Aegilops tauschii in a collection of synthetic hexaploid wheats (Articolo in rivista) (literal)

Anno

2001-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

10.1007/s001220100704 (literal)

Alternative label

Pfluger L.A., D'Ovidio R., Margiotta B., Pena R., Mujeeb-kazi A. ,Lafiandra D. (2001)
Characterization of high and low-molecular weight glutenin subunits associated to the D genome of Aegilops tauschii in a collection of synthetic hexaploid wheats
in Theoretical and applied genetics; Springer Verlag, New York NY (Stati Uniti d'America)
(literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

Pfluger L.A., D'Ovidio R., Margiotta B., Pena R., Mujeeb-kazi A. ,Lafiandra D. (literal)

Pagina inizio

1293 (literal)

Pagina fine

1301 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url

http://biblioproxy.cnr.it:3235/static/pdf/674/art%253A10.1007%252Fs001220100704.pdf?auth66=1387193791_ee701e2933105df2621f88a302b34e46&ext=.pdf (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

103 (literal)

Rivista

Theoretical and applied genetics (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#note

IF 2001 3.438 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

9 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

8 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#descrizioneSinteticaDelProdotto

La combinazione dei risultati ottenuti in SDS-PAGE ed RP-HPLC per le subunità gluteniniche HMW ed LMW ai loci Glu-Dt1 e Glu-Dt3 in accessioni differenti di Aegilops tauschii, ha consentito di identificare tali componenti in questa specie e di rilevare quanto esse siano variabili rispetto ai frumenti coltivati sia in mobilità elettroforetica che in idrofobicità superficiale. L'amplificazione selettiva effettuata mediante PCR, ha inoltre dimostrato che in nessuna delle subunità Dx5t presenti in Aegilops tauschii è presente un residuo extra di cisteina, così come di solito è possibile rilevare nelle subunità Dx5 dei T. aestivum. (literal)

Note

Scopus (literal)
ISI Web of Science (WOS) (literal)
Google Scholar (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

L.A. Pflüger, Instituto de Recursos Biológicos, CIRN, INTA Castelar, (1712) Castelar, Argentina R. D'Ovidio, D. Lafiandra, Dipartimento di Agrobiologia e Agrochimica, Università della Tuscia, Via S. Camillo de Lellis, 01100 Viterbo, Italy B. Margiotta, C.N.R., Istituto di Germoplasma, Bari, Italy R. Peña, A. Mujeeb-Kazi, CIMMYT, Int., Apartado Postal 6-641, 06600 México, D.F., México (literal)

Titolo

Characterization of high and low-molecular weight glutenin subunits associated to the D genome of Aegilops tauschii in a collection of synthetic hexaploid wheats (literal)

Abstract

Synthetic hexaploid wheats (2n=6x=42, AA-BBDD)involving genomes from Triticum turgidum (2n=4x=28, AABB) and Aegilops tauschii (2n=2x=14, DD) have been produced as a means for introducing desirable characteristics into bread wheat. In the present work we describe the genetic variability present at the Glu-Dt1 and Glu-Dt3 loci, encoding high- (HMW) and low-mo-lecular-weight (LMW) glutenin subunits respectively, derived from Ae. tauschii, using electrophoretic and chromatographic methods, in a collection of synthetic hexaploid wheats. A wide variation both in mobility and surface hydrophobicity of HMW glutenin subunits was observed between different accessions of Ae. tauschii used in the production of the synthetic hexaploids. A combination of electrophoretic and chromatographic methods improves the identification of HMW glutenin subunits; in fact subunits with identical apparent mobility were revealed to have a different surface hydrophobicity by reversed-phase high performance liquid chromatography. None of the Dx5 t subunits present in Ae. taus-chii showed the presence of the extra cysteine residue found in the HMW glutenin subunit Dx5 of Triticum aestivum, as revealed by selective amplification with polymerase chain reaction (PCR). The wide variability and the high number of subunits encoded by the Glu-Dt3 locus suggests that Ae. tauschii may be a rich source for enhancing the genetic variability of glutenin subunits in bread wheat and improving bread-making properties. (literal)

Editore