Whispering Gallery Modes Aptasensors for detection of blood proteins (Articolo in rivista)

Type
Label
  • Whispering Gallery Modes Aptasensors for detection of blood proteins (Articolo in rivista) (literal)
Anno
  • 2013-01-01T00:00:00+01:00 (literal)
Alternative label
  • Laura Pasquardini, Simone Berneschi, Andrea Barucci, Franco Cosi, Ramona Dallpiccola, Masimiliano Insinna, Lorenzo Lunelli, Gualtiero Nunzi Conti, Cecilia Pederzolli, Simone Salvadori and Silvia Soria (2013)
    Whispering Gallery Modes Aptasensors for detection of blood proteins
    in Journal of biophotonics (Print)
    (literal)
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  • Laura Pasquardini, Simone Berneschi, Andrea Barucci, Franco Cosi, Ramona Dallpiccola, Masimiliano Insinna, Lorenzo Lunelli, Gualtiero Nunzi Conti, Cecilia Pederzolli, Simone Salvadori and Silvia Soria (literal)
Pagina inizio
  • 178 (literal)
Pagina fine
  • 187 (literal)
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  • 6 (literal)
Rivista
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  • 2 (literal)
Note
  • Scopu (literal)
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  • Fondazione Bruno Kessler, FBK-CMM, Via Sommarive 18, 38123, Trento, Italy Centro Studi e Ricerche E. Fermi, P. Del Viminale 2, 00184 Rome, Italy IFAC-CNR, Institute of Applied Physics, Via Madonna del Piano 10, 50019 Sesto Fiorentino FI, Italy Energy Engineering Dept. S. Stecco, University of Florence, via di S. Marta, 3 50139, Firenze, Italy (literal)
Titolo
  • Whispering Gallery Modes Aptasensors for detection of blood proteins (literal)
Abstract
  • Whispering gallery mode resonators (WGMR), as silica microspheres, have been recently proposed as an efficient tool for the realisation of optical biosensors. In this work we present a functionalization procedure based on the DNA-aptamer sequence immobilization on WGMR, able to recognize specifically thrombin or VEGF protein, preserving a high Q factor. The protein binding was optically characterized in terms of specificity in buffer solution or in 10% diluted human serum. Simulation of the protein flow was found in good agreement with experimental data. The aptasensor was also chemically regenerated and tested again, demonstrating the reusability of our system (literal)
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