Increased levels of Th17 cells are associated with non-neuronal acetylcholine in COPD patients (Articolo in rivista)

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  • Increased levels of Th17 cells are associated with non-neuronal acetylcholine in COPD patients (Articolo in rivista) (literal)
Anno
  • 2014-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1016/j.imbio.2014.01.004 (literal)
Alternative label
  • Mirella Profitaa, Giusy Daniela Albanoa,b, Loredana Riccobonoa, Caterina Di Sanoa, Angela Marina Montalbanoa, Rosalia Gagliardoa, Giulia Anzalonea, Anna Bonannoa, Michael Paul Pieperc, Mark Gjomarkaja (2014)
    Increased levels of Th17 cells are associated with non-neuronal acetylcholine in COPD patients
    in Immunobiology (1979)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Mirella Profitaa, Giusy Daniela Albanoa,b, Loredana Riccobonoa, Caterina Di Sanoa, Angela Marina Montalbanoa, Rosalia Gagliardoa, Giulia Anzalonea, Anna Bonannoa, Michael Paul Pieperc, Mark Gjomarkaja (literal)
Pagina inizio
  • 392 (literal)
Pagina fine
  • 401 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 219 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali
  • 10 (literal)
Note
  • PubMe (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • a Unit: \"Ex vivo/In vitro Models to Study the Immunopathology and the Pharmacology of Airway Diseases\", Institute of Biomedicine and Molecular Immunology (IBIM), Italian National Research Council (CNR), Palermo, Italy b Dipartimento Biomedico di Medicina, Interna e Specialistica (Di.Bi.M.I.S.), Sezione di Pneumologia, University of Palermo, Palermo, Italy c Boehringer Ingelheim Pharma GmbH & Co. KG, Biberach, Germany (literal)
Titolo
  • Increased levels of Th17 cells are associated with non-neuronal acetylcholine in COPD patients (literal)
Abstract
  • T-lymphocytes, including Th17-cells and T-cells expressing acetylcholine (ACh), are key components of systemic inflammation in chronic obstructive pulmonary disease (COPD). We investigated whether ACh promotes Th17 cells in COPD. ACh, IL-17A, IL-22, ROR?t, FOXP3 expression and AChIL-17A, AChIL-22, AChROR?t coexpression was evaluated in peripheral blood mononuclear cells (PBMC) from COPD patients (n=16), healthy smokers (HS) (n=12) and healthy control subjects (HC) (n=13) (cultured for 48h with PMA) by flow cytometry. Furthermore, we studied the effect of Tiotropium (Spiriva®) (100nM) and Olodaterol (1nM) alone or in combination, and of hemicholinium-3 (50?M) on AChIL-17A, AChIL-22, AChROR?t, and FOXP3 expression in CD3+PBT-cells of PBMC from COPD patients (n=6) cultured for 48h with PMA. CD3+PBT-cells expressing ACh, IL-17A, IL-22 and ROR?t together with CD3+PBT-cells co-expressing AChIL-17A, AChIL-22 and AChROR?t were significantly increased in COPD patients compared to HC and HS subjects with higher levels in HS than in HC without a significant difference. CD3+FOXP3+PBT-cells were increased in HS than in HC and COPD. Tiotropium and Olodaterol reduced the percentage of CD3+PBT-cells co-expressing AChIL-17A, AChIL-22, and AChROR?t while increased the CD3+FOXP3+PBT-cells in PBMC from COPD patients, cultured in vitro for 48h, with an additive effect when used in combination. Hemicholnium-3 reduced the percentage of ACh+IL-17A+, ACh+IL-22+, and ACh+ROR?t+ while it did not affect FOXP3+ expression in CD3+PBT-cells from cultured PBMC from COPD patients. We concluded that ACh might promote the increased levels of Th17-cells in systemic inflammation of COPD. Long-acting ?2-agonists and anticholinergic drugs might contribute to control this event. (literal)
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