http://www.cnr.it/ontology/cnr/individuo/prodotto/ID278772
Biomimetic CO2 capture using a highly thermostable bacterial ?-carbonic anhydrase immobilized on a polyurethane foam (Articolo in rivista)
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- Biomimetic CO2 capture using a highly thermostable bacterial ?-carbonic anhydrase immobilized on a polyurethane foam (Articolo in rivista) (literal)
- Anno
- 2014-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.3109/14756366.2012.761608 (literal)
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Fortunato Migliardini1, Viviana De Luca2, Vincenzo Carginale2, Mosè Rossi2,3, Pasquale Corbo1, Claudiu T. Supuran4,5, Clemente Capasso2 (2014)
Biomimetic CO2 capture using a highly thermostable bacterial ?-carbonic anhydrase immobilized on a polyurethane foam
in Journal of enzyme inhibition and medicinal chemistry (Print)
(literal)
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- Fortunato Migliardini1, Viviana De Luca2, Vincenzo Carginale2, Mosè Rossi2,3, Pasquale Corbo1, Claudiu T. Supuran4,5, Clemente Capasso2 (literal)
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- The first two authors contributed equally to this work (literal)
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- 1CNR. Istituto Motori (IM), Via Marconi 8, 80125 Napoli, Italy
2CNR. Istituto di Biochimica delle Proteine (IBP), Via P. Castellino 111, 80131 Napoli, Italy
3Centro di Ricerca Interdipartimentale sui Biomateriali, Università di Napoli, \"Federico II\", P. le V. Tecchio 80, 80125, Napoli, Italy
4Università degli Studi di Firenze, Polo Scientifico, Laboratorio di Chimica Bioinorganica, via della Lastruccia 3, 50019 Sesto Fiorentino, Florence, Italy
5Università degli Studi di Firenze, Polo Scientifico Dipartimento di Scienze Farmaceutiche, 50019 Sesto Fiorentino, Florence, Italy (literal)
- Titolo
- Biomimetic CO2 capture using a highly thermostable bacterial ?-carbonic anhydrase immobilized on a polyurethane foam (literal)
- Abstract
- The biomimetic approach represents an interesting strategy for carbon dioxide (CO2) capture, offering advantages over other methods, due to its specificity for CO2 and its eco-compatibility, as it allows concentration of CO2 from other gases, and its conversion to water soluble ions. This approach uses microorganisms capable of fixing CO2 through metabolic pathways or via the use of an enzyme, such as carbonic anhydrase (CA, EC 4.2.1.1). Recently, our group cloned and purified a novel bacterial ?-CA, named SspCA, from the thermophilic bacteria, Sulfurihydrogenibium yellowstonense YO3AOP1 living in hot springs at temperatures of up to 110 °C. This enzyme showed an exceptional thermal stability, retaining its high catalytic activity for the CO2 hydration reaction even after being heated at 70 °C for several hours. In the present paper, the SspCA was immobilized within a polyurethane (PU) foam. The immobilized enzyme was found to be catalytically active and showed a long-term stability. A bioreactor containing the \"PU-immobilized enzyme\" (PU-SspCA) as shredded foam was used for experimental tests aimed to verify the CO2 capture capability in conditions close to those of a power plant application. In this bioreactor, a gas phase, containing CO2, was put into contact with a liquid phase under conditions, where CO2 contained in the gas phase was absorbed and efficiently converted into bicarbonate by the extremo-?-CA (literal)
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