ATM controls proper mitotic spindle structure. (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• ATM controls proper mitotic spindle structure. (Articolo in rivista) (literal)

Anno

• 2014-01-01T00:00:00+01:00 (literal)

Alternative label

• Palazzo L, Della Monica R, Visconti R, Costanzo V, Grieco D. (2014)
  ATM controls proper mitotic spindle structure.
  in Cell cycle (Georget. Tex.)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Palazzo L, Della Monica R, Visconti R, Costanzo V, Grieco D. (literal)

Rivista

• Cell cycle (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• DMMBM; University of Naples \"Federico II\"; Naples, Italy; Ceinge Biotecnologie Avanzate; Naples, Italy; Current affiliation: Sir William Dunn School of Pathology; University of Oxford; Oxford, UK. DMMBM; University of Naples \"Federico II\"; Naples, Italy; Ceinge Biotecnologie Avanzate; Naples, Italy. IEOS-CNR; Naples, Italy. IFOM; Milan, Italy. (literal)

Titolo

• ATM controls proper mitotic spindle structure. (literal)

Abstract

• The recessive ataxia-telangiectasia (A-T) syndrome is characterized by cerebellar degeneration, immunodeficiency, cancer susceptibility, premature aging, and insulin-resistant diabetes and is caused by loss of function of the ATM kinase, a member of the phosphoinositide 3-kinase-like protein kinases (PIKKs) family. ATM plays a crucial role in the DNA damage response (DDR); however, the complexity of A-T features suggests that ATM may regulate other cellular functions. Here we show that ATM affects proper bipolar mitotic spindle structure independently of DNA damage. In addition, we find that in mitosis ATM forms a complex with the poly(ADP)ribose (PAR) polymerase Tankyrase (TNKS) 1, the spindle pole protein NuMA1, and breast cancer susceptibility protein BRCA1, another crucial DDR player. Our evidence indicates that the complex is required for efficient poly(ADP)ribosylation of NuMA1. We find further that a mutant NuMA1 version, non-phosphorylatable at potential ATM-dependent phosphorylation sites, is poorly PARylated and induces loss of spindle bipolarity. Our findings may help to explain crucial A-T features and provide further mechanistic rationale for TNKS inhibition in cancer therapy. (literal)

Prodotto di

• Institute Experimental Endocrinology and Oncology \"Gaetano Salvatore\" (IEOS) (Istituto)
• Ruolo delle proteine cromatiniche nella tumorigenesi (SV.P15.001.002) (Modulo)

Autore CNR

• ROBERTA VISCONTI (Persona)

Incoming links:

Autore CNR di

• ROBERTA VISCONTI (Persona)

Prodotto

• Institute Experimental Endocrinology and Oncology \"Gaetano Salvatore\" (IEOS) (Istituto)
• Ruolo delle proteine cromatiniche nella tumorigenesi (SV.P15.001.002) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Cell cycle (Rivista)