http://www.cnr.it/ontology/cnr/individuo/prodotto/ID278311

Motility of cryopreserved spermatozoa for the ecotoxicological evaluation of aquatic environments (Articolo in rivista)

Type

Label

Motility of cryopreserved spermatozoa for the ecotoxicological evaluation of aquatic environments (Articolo in rivista) (literal)

Anno

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

Alternative label

Fabbrocini, A., D'Adamo, R., Del Prete, F., Langellotti, A.L., Barone, C.M.A., Rinna, F., Sessa, R., Silvestri, F., Villani, G., Vitiello, V., Sansone, G. (2013)
Motility of cryopreserved spermatozoa for the ecotoxicological evaluation of aquatic environments
in Chemistry in ecology (Print); TAYLOR & FRANCIS LTD, 4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXON (Regno Unito)
(literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

Fabbrocini, A., D'Adamo, R., Del Prete, F., Langellotti, A.L., Barone, C.M.A., Rinna, F., Sessa, R., Silvestri, F., Villani, G., Vitiello, V., Sansone, G. (literal)

Pagina inizio

Pagina fine

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

Rivista

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

CNR, Ist Sci Marine ISMAR, UOS Lesina, Lesina, Italy; Univ Naples Federico II, Dip Biol, Naples, Italy; Univ Naples Federico II, CRIAcq Ctr Interdipartimentale Ric Gest Risorse I, Portici, Italy; Minist Educ Brazil, Capes Fdn, Brasilia, DF, Brazil (literal)

Titolo

Motility of cryopreserved spermatozoa for the ecotoxicological evaluation of aquatic environments (literal)

Abstract

A new approach to environmental studies was investigated by the authors, who propose the use of cryopreserved biological systems in ecotoxicological bioassays. The feasibility of spermiotoxicity tests using cryopreserved semen of the sea bream Sparus aurata, with sperm motility parameters as the endpoint, was evaluated. Thawed sperm was incubated in environmental samples (sediment elutriate and dumpsite leachate) and in a reference toxicant (cadmium) at scaled concentrations. Motility was then evaluated by video-microscopy using both visual and computer-assisted analyses. Activation time, sperm motility and velocity and motility duration were assessed on thawing and at the end of the incubation time, and the difference with respect to the control was statistically evaluated. All the endpoints of the bioassay proved to have good sensitivity even at the highest dilutions of the tested matrices. Observed differences in the sensitivity thresholds of the endpoints were considered to be representative of different aspects of sperm physiology. Therefore the proposed bioassay is a promising starting point for the development of toxicity tests that are increasingly tailored to the needs of ecotoxicology and environmental quality evaluation strategies for aquatic environments. (literal)

Editore