MMP-2 assay within the hybrid layer created by a two-step etch-and-rinse adhesive: Biochemical and immunohistochemical analysis. (Articolo in rivista)

Type
Label
  • MMP-2 assay within the hybrid layer created by a two-step etch-and-rinse adhesive: Biochemical and immunohistochemical analysis. (Articolo in rivista) (literal)
Anno
  • 2011-01-01T00:00:00+01:00 (literal)
Alternative label
  • Mazzoni A, Carrilho M, Papa V, Tjäderhane L, Gobbi P, Nucci C, Di Lenarda R, Mazzotti G, Tay FR, Pashley DH, Breschi L. (2011)
    MMP-2 assay within the hybrid layer created by a two-step etch-and-rinse adhesive: Biochemical and immunohistochemical analysis.
    in Journal of dentistry
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Mazzoni A, Carrilho M, Papa V, Tjäderhane L, Gobbi P, Nucci C, Di Lenarda R, Mazzotti G, Tay FR, Pashley DH, Breschi L. (literal)
Pagina inizio
  • 470 (literal)
Pagina fine
  • 477 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 39 (literal)
Rivista
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Department of SAU&FAL, University of Bologna, Bologna, Italy; Laboratory of Cell Biology & Laboratory of Immunorheumatology and Tissue Regeneration - Ramses Laboratory, c/o Rizzoli Orthopaedic Institute, Bologna, Italy; Bandeirante University of Sao Paulo (UNIBAN), Sao Paulo, Brazil; Department of Restorative Dentistry, Piracicaba School of Dentistry, University of Campinas, Piracicaba, Brazil; Department of Sport and Health Sciences, University of Cassino, Cassino, Italy; Institute of Dentistry, University of Oulu, Oulu University Hospital (OUH), Oulu, Finland; Department of STeVA, University ‘‘Carlo Bo’’, Urbino, Italy; Department of Dental Sciences, University of Bologna, Bologna, Italy; Department of Medical Sciences, Unit of Dental Sciences and Biomaterials, University of Trieste, Trieste, Italy; Department of Oral Biology, College of Dental Medicine, Georgia Health Sciences University, Augusta, GA, USA; IGM-CNR, Unit of Bologna, c/o IOR, Bologna, Italy (literal)
Titolo
  • MMP-2 assay within the hybrid layer created by a two-step etch-and-rinse adhesive: Biochemical and immunohistochemical analysis. (literal)
Abstract
  • OBJECTIVE: Degradation of hybrid layers (HLs) within resin-infiltrated dentine results from multiple degradation factors, including collagenolytic activity of specific matrix metalloproteinases (MMPs). Inhibition of host-derived MMPs may, therefore, slow the degradation of HL. The null hypothesis tested is that the presence of MMP-2 is similar regardless of chlorhexidine (CHX) pre-treatment or the use of an adhesive. METHODS: Powdered dentine prepared from extracted human teeth was divided into 4 groups: (G1) mineralised powder (control group); (G2) dentine powder treated with 1% phosphoric acid for 1 min; (G3) 1% phosphoric acid-etched dentine treated with Adper Scotchbond 1 XT (SB1XT; 3M ESPE); (G4) 1% phosphoric acid-etched dentine treated with 0.2% CHX followed by SB1XT. The concentration of detectable pro-MMP-2 and MMP-2 was assayed using a colorimetric assay system (QuantiSir). In addition, the presence of MMP-2 in the HL was assessed in 1 year-aged adhesive-dentine interfaces using an immunohistochemical approach under FEI-SEM/TEM. RESULTS: In dentine powder treated with 1% phosphoric acid (G2), MMP-2 level decreased compared to controls (G1); the application of SB1XT (G3) resulted in an increase of MMP-2, whilst 0.2% CHX before SB1XT application (G4), reduced MMP-2. The FEI-SEM/TEM analysis revealed MMP-2 distribution within the HL of aged interfaces showing increase MMP-2 patterns in the control group and minor labelling in the CHX-pretreated specimens. CONCLUSION: The results of this study support the use of non-toxic MMPs inhibitors, such as CHX, as an appropriate additional step in bonding procedures in order to increase the longevity of the adhesive restorations. (literal)
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