Functional analysis of a murine monoclonal antibody against the repetitive region of the fibronectin-binding adhesins fibronectin-binding protein A and fibronectin-binding protein B from Staphylococcus aureus. (Articolo in rivista)

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  • Functional analysis of a murine monoclonal antibody against the repetitive region of the fibronectin-binding adhesins fibronectin-binding protein A and fibronectin-binding protein B from Staphylococcus aureus. (Articolo in rivista) (literal)
Anno
  • 2010-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1111/j.1742-4658.2010.07835.x (literal)
Alternative label
  • Provenza G;1 Provenzano M;1 Visai L;2 Burke FM;3 Geoghegan JA;3 Stravalaci M;4 Gobbi M;4 Mazzini G;5 Arciola CR;6 Foster TJ;3 Speziale P.1 (2010)
    Functional analysis of a murine monoclonal antibody against the repetitive region of the fibronectin-binding adhesins fibronectin-binding protein A and fibronectin-binding protein B from Staphylococcus aureus.
    in The FEBS journal (Print)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Provenza G;1 Provenzano M;1 Visai L;2 Burke FM;3 Geoghegan JA;3 Stravalaci M;4 Gobbi M;4 Mazzini G;5 Arciola CR;6 Foster TJ;3 Speziale P.1 (literal)
Pagina inizio
  • 4490 (literal)
Pagina fine
  • 4505 (literal)
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  • http://www.cnr.it (literal)
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  • 277 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
  • 21 (literal)
Note
  • ISI Web of Science (WOS) (literal)
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  • 1. Univ Pavia, Dept Biochem, I-27100 Pavia, Italy 2. Univ Pavia, Ctr Tissue Engn CIT, I-27100 Pavia, Italy 3. Univ Dublin, Dept Microbiol, Moyne Inst Prevent Med, Dublin, Ireland 4. Ist Ric Farmacol Mario Negri, Dept Biochem & Mol Pharmacol, Milan, Italy 5. Univ Pavia, Dept Anim Biol, IGM CNR Histochem & Cytometry, I-27100 Pavia, Italy 6. Rizzoli Orthoped Inst, Res Unit Implant Infect, Bologna, Italy (literal)
Titolo
  • Functional analysis of a murine monoclonal antibody against the repetitive region of the fibronectin-binding adhesins fibronectin-binding protein A and fibronectin-binding protein B from Staphylococcus aureus. (literal)
Abstract
  • Fibronectin-binding proteins A and B are multifunctional LPXTG staphylococcal adhesins, comprising an N-terminal region that binds fibrinogen and elastin, and a C-terminal domain that interacts with fibronectin. The C-terminal domain of fibronectin-binding protein A is organized into 11 tandem repeats, six of which bind the ligand with high affinity; other sites bind more weakly. Fibronectin-binding protein B has been postulated to harbor 10 rather than 11 repeats, but it contains the same number of high-affinity fibronectin-binding sites as fibronectin-binding protein A. In this study, we confirm this prediction and show that six of 10 sites bind with dissociation constants in the nanomolar range. We also found that the full-length repetitive region of fibronectin-binding protein B stimulated the production of a mAb (15E11) that binds with high affinity to an epitope shared by repeats 9 and 10 from both adhesins. With the use of truncated fragments of repeat 9 of fibronectin-binding protein A, we mapped the antibody epitope to the N-terminal segment and the fibronectin-binding site to the C-terminal segment of the repeat. The distinct localization of the 15E11 epitope and the fibronectin-binding site suggests that the interfering effect of the antibody might result from steric hindrance or a conformational change in the structure that reduces the accessibility of fibronectin to its binding determinant. The epitope is well exposed on the surface of staphylococcal cells, as determined by genetic analyses, fluorescence microscopy, and flow cytometry. When incubated with cells of Staphylococcus aureus strains, 15E11 inhibits attachment of bacteria to surface-coated fibronectin by almost 70%. (literal)
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